Identification and characterization of ...
Type de document :
Article dans une revue scientifique
URL permanente :
Titre :
Identification and characterization of miRNAs and their potential targets in flax
Auteur(s) :
Neutelings, Godfrey [Auteur]
Université Lille Nord de France (COMUE)
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Fénart, Stéphane [Auteur]
Lucau, Anca [Auteur]
Université Lille Nord de France (COMUE)
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Hawkins, Simon [Auteur]
Université Lille Nord de France (COMUE)
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Université Lille Nord de France (COMUE)
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Fénart, Stéphane [Auteur]
Lucau, Anca [Auteur]
Université Lille Nord de France (COMUE)
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Hawkins, Simon [Auteur]
Université Lille Nord de France (COMUE)
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Titre de la revue :
Journal of Plant Physiology
Numéro :
169
Pagination :
1754-1766
Éditeur :
Elsevier BV
Date de publication :
2012-11-15
ISSN :
0176-1617
Mot(s)-clé(s) en anglais :
Flax
Gene regulation
MicroRNAs
Stem-loop structure
Target mRNAs
Gene regulation
MicroRNAs
Stem-loop structure
Target mRNAs
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
MicroRNAs (miRNAs) are small non-protein coding regulatory RNAs released after the cleavage of a primary transcript. A computational homology search of expressed sequence tags (ESTs) available in public databases allowed ...
Lire la suite >MicroRNAs (miRNAs) are small non-protein coding regulatory RNAs released after the cleavage of a primary transcript. A computational homology search of expressed sequence tags (ESTs) available in public databases allowed the identification of 20 conserved miRNAs belonging to 13 different families in flax (Linum usitatissimum). Most of the miRNAs were 21 nucleotides-long and carried a uracil at the 5′ end. They originated from precursor transcripts that vary greatly in length. A single precursor containing 2 different stem-loop structures, each one carrying a member of the miR398 family, was identified for the first time in plants. qRT-PCR analyses of 4 selected miRNAs indicated that all were differentially expressed in flax tissues. The 20 miRNAs could potentially regulate 112 different targets including genes involved in cell wall metabolism. Analyses of pri-miRNA and potential gene expression profiles in a publically available microarray data set allowed the identification of a number of highly opposite pri-miRNA/target gene profiles potentially involved in regulating plantacyanin levels, F-box mediated signalling processes, protein metabolism and ion homeostasis, as well as 6 unknown processes.Lire moins >
Lire la suite >MicroRNAs (miRNAs) are small non-protein coding regulatory RNAs released after the cleavage of a primary transcript. A computational homology search of expressed sequence tags (ESTs) available in public databases allowed the identification of 20 conserved miRNAs belonging to 13 different families in flax (Linum usitatissimum). Most of the miRNAs were 21 nucleotides-long and carried a uracil at the 5′ end. They originated from precursor transcripts that vary greatly in length. A single precursor containing 2 different stem-loop structures, each one carrying a member of the miR398 family, was identified for the first time in plants. qRT-PCR analyses of 4 selected miRNAs indicated that all were differentially expressed in flax tissues. The 20 miRNAs could potentially regulate 112 different targets including genes involved in cell wall metabolism. Analyses of pri-miRNA and potential gene expression profiles in a publically available microarray data set allowed the identification of a number of highly opposite pri-miRNA/target gene profiles potentially involved in regulating plantacyanin levels, F-box mediated signalling processes, protein metabolism and ion homeostasis, as well as 6 unknown processes.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
CNRS
CNRS
Date de dépôt :
2021-01-06T16:26:08Z
2021-01-07T15:01:53Z
2021-01-07T15:01:53Z
Fichiers
- [2012] Neutelings et al.pdf
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