Title :

Chitosan/polycyclodextrin (cht/pcd)-based sponges delivering vegf to enhance angiogenesis for bone regeneration

Author(s) :

Palomino Durand, Carla [Auteur]
Physiopathologie des Maladies Osseuses Inflammatoires (PMOI) - ULR 4490
Lopez, Marco [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Marchandise, Pierre [Auteur]
Unité de Taphonomie Médico-Légale (UTML) - ULR 7367
Martel, Bernard [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Unité Matériaux et Transformations (UMET) - UMR 8207
Blanchemain, Nicolas [Auteur]
Advanced Drug Delivery Systems (ADDS) - U1008
Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS]
Chai, Feng [Auteur]

Journal title :

Pharmaceutics

Abbreviated title :

Pharmaceutics

Volume number :

12

Pages :

784

Publisher :

MDPI

Publication date :

2020-08-19

ISSN :

1999-4923

Keyword(s) :

sponges
VEGF-delivery
cyclodextrin polymer
cell proliferation
cell migration
bone tissue engineering
chitosan

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Vascularization is one of the main challenges in bone tissue engineering (BTE). In this study, vascular endothelial growth factor (VEGF), known for its angiogenic effect, was delivered by our developed sponge, derived from ...
Show more >Vascularization is one of the main challenges in bone tissue engineering (BTE). In this study, vascular endothelial growth factor (VEGF), known for its angiogenic effect, was delivered by our developed sponge, derived from a polyelectrolyte complexes hydrogel between chitosan (CHT) and anionic cyclodextrin polymer (PCD). This sponge, as a scaffold for growth factor delivery, was formed by freeze-drying a homogeneous CHT/PCD hydrogel, and thereafter stabilized by a thermal treatment. Microstructure, water-uptake, biodegradation, mechanical properties, and cytocompatibility of sponges were assessed. VEGF-delivery following incubation in medium was then evaluated by monitoring the VEGF-release profile and its bioactivity. CHT/PCD sponge showed a porous (open porosity of 87.5%) interconnected microstructure with pores of different sizes (an average pore size of 153 μm), a slow biodegradation (12% till 21 days), a high water-uptake capacity (~600% in 2 h), an elastic property under compression (elastic modulus of compression 256 ± 4 kPa), and a good cytocompatibility in contact with osteoblast and endothelial cells. The kinetic release of VEGF was found to exert a pro-proliferation and a pro-migration effect on endothelial cells, which are two important processes during scaffold vascularization. Hence, CHT/PCD sponges were promising vehicles for the delivery of growth factors in BTEShow less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
CNRS
ENSCL
INRA
Inserm
Université de Lille

Collections :

• Advanced Drug Delivery Systems (ADDS) - U1008
• Unité Matériaux et Transformations (UMET) - UMR 8207

Submission date :

2021-01-20T15:59:28Z
2022-11-09T07:28:59Z