Reverse line blot hybridisation screening ...
Document type :
Compte-rendu et recension critique d'ouvrage
PMID :
Title :
Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis
Author(s) :
Lu, Q. [Auteur]
Peking University [Beijing]
van den Ende, A.-H.-G. Gerrits [Auteur]
de Hoog, G. Sybren [Auteur correspondant]
Peking University [Beijing]
University of Amsterdam [Amsterdam] = Universiteit van Amsterdam [UvA]
Li, R. [Auteur]
Peking University [Beijing]
Accoceberry, Isabelle [Auteur]
Hôpital Saint-André
Durand-Joly, Isabelle [Auteur]
Bouchara, Jean-Philippe [Auteur]
Groupe d'Étude des Interactions Hôte-Pathogène [GEIHP]
Hernandez, F. [Auteur]
Service de Parasitologie-Mycologie [CHRU LIlle]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Delhaès, Laurence [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Service de Parasitologie-Mycologie [CHRU LIlle]
Peking University [Beijing]
van den Ende, A.-H.-G. Gerrits [Auteur]
de Hoog, G. Sybren [Auteur correspondant]
Peking University [Beijing]
University of Amsterdam [Amsterdam] = Universiteit van Amsterdam [UvA]
Li, R. [Auteur]
Peking University [Beijing]
Accoceberry, Isabelle [Auteur]
Hôpital Saint-André
Durand-Joly, Isabelle [Auteur]
Bouchara, Jean-Philippe [Auteur]
Groupe d'Étude des Interactions Hôte-Pathogène [GEIHP]
Hernandez, F. [Auteur]
Service de Parasitologie-Mycologie [CHRU LIlle]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Delhaès, Laurence [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Service de Parasitologie-Mycologie [CHRU LIlle]
Journal title :
Mycoses
Pages :
5 - 11
Publication date :
2011-10-14
ISSN :
1439-0507
English keyword(s) :
Cystic fibrosis
Pseudallescheria
reverse line blot hybridisation
Scedosporium
Pseudallescheria
reverse line blot hybridisation
Scedosporium
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum ...
Show more >The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.Show less >
Show more >The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.Show less >
Language :
Anglais
Popular science :
Non
Source :