Rapid and sensitive identification of ...
Document type :
Article dans une revue scientifique
Permalink :
Title :
Rapid and sensitive identification of uropathogenic Escherichia coli using a surface-enhanced-Raman-scattering-based biochip
Author(s) :
Andrei, Cristina-Cassiana [Auteur]
Moraillon, Anne [Auteur]
Lau, Stephanie [Auteur]
Felidj, Nordin [Auteur]
Yamakawa, Nao [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Larquet, Eric [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Ozanam, François [Auteur]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Chantal Gouget-Laemmel, Anne [Auteur]
Moraillon, Anne [Auteur]
Lau, Stephanie [Auteur]
Felidj, Nordin [Auteur]
Yamakawa, Nao [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Bouckaert, Julie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Larquet, Eric [Auteur]
Boukherroub, Rabah [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Ozanam, François [Auteur]
Szunerits, Sabine [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Chantal Gouget-Laemmel, Anne [Auteur]
Journal title :
Talanta
Volume number :
219
Pages :
121174
Publisher :
Elsevier BV
Publication date :
2020-11-01
ISSN :
0039-9140
English keyword(s) :
Biochips
Surface-enhanced Raman spectroscopy (SERS)
Gold nanorods (Au NRs)
Escherichia coli
Principle component analysis (PCA)
Hydrogenated amorphous silicon (a-Si:H)
Surface-enhanced Raman spectroscopy (SERS)
Gold nanorods (Au NRs)
Escherichia coli
Principle component analysis (PCA)
Hydrogenated amorphous silicon (a-Si:H)
HAL domain(s) :
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Rapid, selective and sensitive sensing of bacteria remains challenging. We report on a highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS)-based sensing approach for the detection of uropathogenic ...
Show more >Rapid, selective and sensitive sensing of bacteria remains challenging. We report on a highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS)-based sensing approach for the detection of uropathogenic Escherichia coli (E. coli) bacteria in urine. The assay is based on the specific capture of the bacteria followed by interaction with cetyltrimethylammonium bromide (CTAB)-stabilised gold nanorods (Au NRS) as SERS markers. High sensitivity up to 10 CFU mL-1 is achieved by optimizing the capture interface based on hydrogenated amorphous silicon a-Si:H thin films. The integration of CH3O-PEG750 onto a-Si:H gives the sensing interface an efficient anti-fouling character, while covalent linkage of antibodies directed against the major type-1 fimbrial pilin FimA of the human pathogen E. coli results in the specific trapping of fimbriated E. coli onto the SERS substrate and their spectral fingerprint identification.Show less >
Show more >Rapid, selective and sensitive sensing of bacteria remains challenging. We report on a highly sensitive and reproducible surface-enhanced Raman spectroscopy (SERS)-based sensing approach for the detection of uropathogenic Escherichia coli (E. coli) bacteria in urine. The assay is based on the specific capture of the bacteria followed by interaction with cetyltrimethylammonium bromide (CTAB)-stabilised gold nanorods (Au NRS) as SERS markers. High sensitivity up to 10 CFU mL-1 is achieved by optimizing the capture interface based on hydrogenated amorphous silicon a-Si:H thin films. The integration of CH3O-PEG750 onto a-Si:H gives the sensing interface an efficient anti-fouling character, while covalent linkage of antibodies directed against the major type-1 fimbrial pilin FimA of the human pathogen E. coli results in the specific trapping of fimbriated E. coli onto the SERS substrate and their spectral fingerprint identification.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Collections :
Research team(s) :
Computational Molecular Systems Biology
Submission date :
2021-03-23T13:51:19Z
2021-03-23T15:49:13Z
2021-03-23T16:26:36Z
2021-03-23T15:49:13Z
2021-03-23T16:26:36Z
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