Titre :

Isolation of microglia-derived extracellular vesicles: towards mirna signatures and neuroprotection

Auteur(s) :

Lemaire, Quentin [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Raffo Romero, Antonella [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Arab, Tanina [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Van Camp, Christelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Forte, Stefano [Auteur]
Gimeno, Jean-Pascal [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Doye, Séverine [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Colin, Morvane [Auteur]
Lille Neurosciences & Cognition - U 1172 [LilNCog]
Vizioli, Jacopo [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Sautiere, Pierre-Eric [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Lefebvre, Christophe [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 [PRISM]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Titre de la revue :

Journal of nanobiotechnology

Nom court de la revue :

J Nanobiotechnology

Numéro :

17

Pagination :

119

Date de publication :

2019-12-04

ISSN :

1477-3155

Mot(s)-clé(s) en anglais :

miRNAs
Extracellular vesicles
Microglia
Neuroprotection
Leech Hirudo medicinalis

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

The functional preservation of the central nervous system (CNS) is based on the neuronal plasticity and survival. In this context, the neuroinflammatory state plays a key role and involves the microglial cells, the ...
Lire la suite >The functional preservation of the central nervous system (CNS) is based on the neuronal plasticity and survival. In this context, the neuroinflammatory state plays a key role and involves the microglial cells, the CNS‑resident macrophages. In order to better understand the microglial contribution to the neuroprotection, microglia‑derived extracellular vesicles (EVs) were isolated and molecularly characterized to be then studied in neurite outgrowth assays. The EVs, mainly composed of exosomes and microparticles, are an important cell‑to‑cell communication process as they exhibit different types of mediators (proteins, lipids, nucleic acids) to recipient cells. The medicinal leech CNS was initially used as an interesting model of microglia/neuron crosstalk due to their easy collection for primary cultures. After the microglia‑derived EV isolation following successive methods, we developed their large‑scale and non‑ targeted proteomic analysis to (i) detect as many EV protein markers as possible, (ii) better understand the biologically active proteins in EVs and (iii) evaluate the resulting protein signatures in EV‑activated neurons. The EV functional properties were also evaluated in neurite outgrowth assays on rat primary neurons and the RNAseq analysis of the microglia‑derived EVs was performed to propose the most representative miRNAs in microglia‑derived EVs. This strategy allowed validating the EV isolation, identify major biological pathways in EVs and corroborate the regenera‑ tive process in EV‑activated neurons. In parallel, six different miRNAs were originally identified in microglia‑derived EVs including 3 which were only known in plants until now. The analysis of the neuronal proteins under the microglial EV activation suggested possible miRNA‑dependent regulation mechanisms. Taken together, this combination of methodologies showed the leech microglial EVs as neuroprotective cargos across species and contributed to pro‑ pose original EV‑associated miRNAs whose functions will have to be evaluated in the EV‑dependent dialog between microglia and neurons.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

CHU Lille
CNRS
INSERM
Inserm
Université de Lille

Collections :

• Lille Neurosciences & Cognition (LilNCog) - U 1172
• Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192

Équipe(s) de recherche :

Alzheimer et Tauopathies

Date de dépôt :

2021-06-23T13:45:13Z
2024-02-23T09:08:22Z