Title :

Antibodies against hla cross-reactivity groups: from single antigen bead assay to immunoinformatics interpretation of epitopes

Author(s) :

Usureau, Cedric [Auteur]
Lefevre, Edgar [Auteur]
Top, Isabelle [Auteur]
Nikolski, Macha [Auteur]
Varlet, Pauline [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Choukroun, Gabriel [Auteur]
Desoutter, Judith [Auteur]
Guillaume, Nicolas [Auteur]

Journal title :

Molecular immunology

Abbreviated title :

Mol Immunol

Volume number :

133

Pages :

154-162

Publication date :

2021-03-02

ISSN :

1872-9142

Keyword(s) :

Transplantation
Antibody
Epitope
Major histocompatibility complex

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Identification of anti-human leukocyte antigen (HLA) antibodies (Abs) is based on Luminex™ technology. We used bioinformatics to (i) study the correlations of mean fluorescence intensities (MFIs) for all the possible allele ...
Show more >Identification of anti-human leukocyte antigen (HLA) antibodies (Abs) is based on Luminex™ technology. We used bioinformatics to (i) study the correlations of mean fluorescence intensities (MFIs) for all the possible allele pairs, and (ii) determine the degree of epitope homology between HLA antigens. Using MFI data on anti-HLA Abs from 6000 Luminex™ assays, we provide an updated overview of class I and II HLA antigen cross-reactivity in which each node corresponded to an allele and each link corresponded to a strong correlation between two alleles (Spearman's ρ > 0.8). We compared these correlations with the serological groups and the results of an epitope analysis. The strongest correlations concerned allele-specific Abs directed against the same antigen. For the HLA-A locus, the highest values of Spearman's ρ reflected broad specificity. For the HLA-B locus, graphs defined the HLA-Bw4 public epitope, and correlations between HLA-A and -B alleles were only present for beads with the same Bw4 public epitope. For the HLA-C locus, we identified two groups that differed with regard to their KIR ligand subclassification. Lastly, the HLA-DRB1 subgroups were part of a network. In the epitope analysis, Spearman's ρ was related to the number of matched epitopes within pairs of alleles. The combination of Spearman's ρ with simple, undirected graphing constitutes an effective tool for understanding routinely encountered cross-reactivity profiles. Based on this model, we have implemented an online data visualization tool available at http://cusureau.pythonanywhere.com/.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2021-07-06T12:50:02Z