Identification of cell wall proteins in ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
Title :
Identification of cell wall proteins in the flax (Linum usitatissimum) stem
Author(s) :
Day, Arnaud [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Fenart, Stephane [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Neutelings, Godfrey [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Hawkins, Simon [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Rolando, Christian [Auteur]
Université de Lille, Sciences et Technologies
Centre National de la Recherche Scientifique [CNRS]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Tokarski, Caroline [Auteur]
Université de Lille, Sciences et Technologies
Centre National de la Recherche Scientifique [CNRS]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Fenart, Stephane [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Neutelings, Godfrey [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Hawkins, Simon [Auteur]
Stress Abiotiques et Différenciation des Végétaux Cultivés [SADV]
Centre National de la Recherche Scientifique [CNRS]
Rolando, Christian [Auteur]
Université de Lille, Sciences et Technologies
Centre National de la Recherche Scientifique [CNRS]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Tokarski, Caroline [Auteur]
Université de Lille, Sciences et Technologies
Centre National de la Recherche Scientifique [CNRS]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Journal title :
Proteomics
Pages :
812 - 825
Publisher :
Wiley-VCH Verlag
Publication date :
2013
ISSN :
1615-9853
Keyword(s) :
Flax
Plant proteomics
PROTEOMICS
L.
Plant proteomics
PROTEOMICS
L.
English keyword(s) :
Cell wall
FT-ICR high resolution MS
PLANT GLYCOSIDE HYDROLASES
GERMIN-LIKE PROTEIN
PECTIN METHYLESTERASE
ARABIDOPSIS-THALIANA
PHYSIOLOGICAL ROLES
ELONGATING CELLS
BAST FIBERS
PEROXIDASE
FT-ICR high resolution MS
PLANT GLYCOSIDE HYDROLASES
GERMIN-LIKE PROTEIN
PECTIN METHYLESTERASE
ARABIDOPSIS-THALIANA
PHYSIOLOGICAL ROLES
ELONGATING CELLS
BAST FIBERS
PEROXIDASE
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biologie végétale
Sciences du Vivant [q-bio]/Biologie végétale
English abstract : [en]
Sequential salt (CaCl2, LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use ...
Show more >Sequential salt (CaCl2, LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use of recently published genomic data allowed the identification of 11912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using TargetP, Predotar, and WoLF PSORT led to the identification of 152 putative flax CWPs that were classified into nine different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell-wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWPs in flax and will lead to a better understanding of cell wall biology in this species.Show less >
Show more >Sequential salt (CaCl2, LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use of recently published genomic data allowed the identification of 11912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using TargetP, Predotar, and WoLF PSORT led to the identification of 152 putative flax CWPs that were classified into nine different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell-wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWPs in flax and will lead to a better understanding of cell wall biology in this species.Show less >
Language :
Anglais
Popular science :
Non
Source :
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