Title :

First identification of Cryptosporidium parvum zoonotic subtype IIaA15G2R1 in diarrheal lambs in France

Author(s) :

Mammeri, Mohamed [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Cartou, Lara [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Julien, Christine [Auteur]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Yncréa Hauts-de-France
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Adjou, Karim Tarik [Auteur correspondant]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]

Journal title :

Veterinary Parasitology : Regional Studies and Reports

Publisher :

Elsevier

Publication date :

2019-12

ISSN :

2405-9390

English keyword(s) :

Cryptosporidium
Lamb
Sheep
Zoonosis
France

HAL domain(s) :

Sciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animale

English abstract : [en]

To date, no information is available about the presence of Cryptosporidium spp. in French sheep, nor their potential role as zoonotic reservoirs. A total of 23 fecal samples were collected from diarrheic lambs (< 11 days ...
Show more >To date, no information is available about the presence of Cryptosporidium spp. in French sheep, nor their potential role as zoonotic reservoirs. A total of 23 fecal samples were collected from diarrheic lambs (< 11 days old) from seven randomly selected farms. Cryptosporidium-oocysts were detected microscopically with Direct Immunofluorescence Assays (DFA) in 23/23 (100%) of fecal samples. PCR-RFLP of the 18S rRNA gene was used to determine species in all samples, and only Cryptosporidium parvum was identified. Isolates were subtyped by sequencing the 60 kDa glycoprotein (gp60) gene. Two zoonotic subtypes within the IIa subtype family were identified, including IIaA15G2R1 (22/23) and IIaA16G3R1 (1/23). This study reports for the first time the identification and genotyping of zoonotic C. parvum subtypes from lambs in France. Sheep could thus play an important role as potential reservoirs for this zoonotic protist.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL