Identification of proteins involved in the ...
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Article dans une revue scientifique: Article original
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Title :
Identification of proteins involved in the anti-inflammatory properties of Propionibacterium freudenreichii by means of a multi-strain study
Author(s) :
Deutsch, Stephanie-Marie [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Mariadassou, Mahendra [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Nicolas, Pierre [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Parayre, Sandrine [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Le Guellec, Rozenn [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Chuat, Victoria [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Peton, Vincent [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Le Marechal, Caroline [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Burati, Julien [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Loux, Valentin [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Briard-Bion, Valerie [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Jardin, Julien [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Ple, Coline [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Foligne, Benoit [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Jan, Gwenael [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Falentin, Helene [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Science et Technologie du Lait et de l'Oeuf [STLO]
Mariadassou, Mahendra [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Nicolas, Pierre [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Parayre, Sandrine [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Le Guellec, Rozenn [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Chuat, Victoria [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Peton, Vincent [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Le Marechal, Caroline [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Burati, Julien [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Loux, Valentin [Auteur]
Mathématiques et Informatique Appliquées du Génome à l'Environnement [Jouy-En-Josas] [MaIAGE]
Briard-Bion, Valerie [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Jardin, Julien [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Ple, Coline [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Foligne, Benoit [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Jan, Gwenael [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Falentin, Helene [Auteur]
Science et Technologie du Lait et de l'Oeuf [STLO]
Journal title :
Scientific Reports
Abbreviated title :
Sci Rep
Volume number :
7
Publication date :
2017-04-13
ISSN :
2045-2322
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent ...
Show more >Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties.Show less >
Show more >Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics. The omics data were then correlated to the anti-inflammatory potential evaluated by IL-10 induction. This comparative omics strategy highlighted candidate genes that were further subjected to gene-inactivation validation. This validation confirmed the contribution of surface proteins, including SlpB and SlpE, two proteins with SLH domains known to mediate non-covalent anchorage to the cell-wall. Interestingly, HsdM3, predicted as cytoplasmic and involved in DNA modification, was shown to contribute to anti-inflammatory activity. Finally, we demonstrated that a single protein cannot explain the anti-inflammatory properties of a strain. These properties therefore result from different combinations of surface and cytoplasmic proteins, depending on the strain. Our enhanced understanding of the molecular bases for immunomodulation will enable the relevant screening for bacterial resources with anti-inflammatory properties.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Inserm
Université de Lille
CHU Lille
Université de Lille
CHU Lille
Research team(s) :
Inflammatory digestive disease : pathophysiology and therapeutic targets developement
Submission date :
2019-03-01T14:09:34Z
2022-07-06T09:30:57Z
2022-07-06T09:30:57Z
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