A standardized flow cytometry procedure ...
Type de document :
Article dans une revue scientifique
DOI :
PMID :
URL permanente :
Titre :
A standardized flow cytometry procedure for the monitoring of regulatory T cells in clinical trials.
Auteur(s) :
Pitoiset, Fabien [Auteur]
Immunologie - Immunopathologie - Immunothérapie [I3]
Barbie, Michele [Auteur]
Immunologie - Immunopathologie - Immunothérapie [I3]
Monneret, Guillaume [Auteur]
Braudeau, Cecile [Auteur]
Centre de Recherche en Transplantation et Immunologie [U1064 Inserm - CRTI]
Pochard, Pierre [Auteur]
Immunologie et Pathologie [EA2216]
Pellegrin, Isabelle [Auteur]
Trauet, Jacques [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Labalette, Myriam [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Klatzmann, David [Auteur]
Immunologie - Immunopathologie - Immunothérapeutique [I3]
Rosenzwajg, Michelle [Auteur]
Immunologie - Immunopathologie - Immunothérapeutique [I3]
Immunologie - Immunopathologie - Immunothérapie [I3]
Barbie, Michele [Auteur]
Immunologie - Immunopathologie - Immunothérapie [I3]
Monneret, Guillaume [Auteur]
Braudeau, Cecile [Auteur]
Centre de Recherche en Transplantation et Immunologie [U1064 Inserm - CRTI]
Pochard, Pierre [Auteur]
Immunologie et Pathologie [EA2216]
Pellegrin, Isabelle [Auteur]
Trauet, Jacques [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Labalette, Myriam [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Klatzmann, David [Auteur]
Immunologie - Immunopathologie - Immunothérapeutique [I3]
Rosenzwajg, Michelle [Auteur]
Immunologie - Immunopathologie - Immunothérapeutique [I3]
Titre de la revue :
Cytometry Part B: Clinical Cytometry
Nom court de la revue :
Cytometry B Clin Cytom
Numéro :
94
Pagination :
777-782
Date de publication :
2018-09
Mot(s)-clé(s) :
flow cytometry
regulatory T cells
standardization
regulatory T cells
standardization
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Background: Quantification of regulatory T cells (Tregs) is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune ...
Lire la suite >Background: Quantification of regulatory T cells (Tregs) is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune diseases, infections, and allergies. Human Tregs are defined as CD4+ CD25+ CD127low FoxP3+ cells, and the standardization of their staining by flow cytometry is a challenge, especially in multicenter clinical trials, notably because of the intracellular location of FoxP3. Method: A flow cytometry staining procedure was settled and standardized to measure human Tregs in peripheral whole blood using precoated dried antibodies in ready-to-use tubes. It was compared with reference methods and implemented and validated to be suitable with different cytometer platforms. Results: The standardized protocol developed with dried antibodies and reduced volumes of whole blood allows an optimal identification of Tregs. Compared with classical staining procedure, it reduces the number of steps required, in a very fast and simple technique. The accuracy of the method was confirmed by a multicenter comparison with different cytometer brands. Conclusions: Our results highlight the reliability of this high-standard protocol that could become a reference method for the monitoring of Tregs in clinical trials. © 2018 International Clinical Cytometry SocietyLire moins >
Lire la suite >Background: Quantification of regulatory T cells (Tregs) is crucial in immunomonitoring in clinical trials as this cell population has been shown to be involved in a wide range of diseases, including cancers, autoimmune diseases, infections, and allergies. Human Tregs are defined as CD4+ CD25+ CD127low FoxP3+ cells, and the standardization of their staining by flow cytometry is a challenge, especially in multicenter clinical trials, notably because of the intracellular location of FoxP3. Method: A flow cytometry staining procedure was settled and standardized to measure human Tregs in peripheral whole blood using precoated dried antibodies in ready-to-use tubes. It was compared with reference methods and implemented and validated to be suitable with different cytometer platforms. Results: The standardized protocol developed with dried antibodies and reduced volumes of whole blood allows an optimal identification of Tregs. Compared with classical staining procedure, it reduces the number of steps required, in a very fast and simple technique. The accuracy of the method was confirmed by a multicenter comparison with different cytometer brands. Conclusions: Our results highlight the reliability of this high-standard protocol that could become a reference method for the monitoring of Tregs in clinical trials. © 2018 International Clinical Cytometry SocietyLire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Inserm
Université de Lille
CHU Lille
Université de Lille
CHU Lille
Équipe(s) de recherche :
Immunity, inflammation and fibrsis in auto and allo-reactivity
Date de dépôt :
2019-03-01T14:25:46Z
2024-02-02T09:27:18Z
2024-02-02T09:27:18Z
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