Title :

Concerted mass spectrometry-based glycomic approach for precision mapping of sulfo sialylated N-glycans on human peripheral blood mononuclear cells and lymphocytes

Author(s) :

Chen, Jian-You [Auteur]
National Taïwan University [NTU]
Huang, Hsin-Hung [Auteur]
National Taïwan University [NTU]
Yu, Shin-Yi [Auteur]
Lille Neurosciences & Cognition (LilNCog) - U 1172
Lille Inflammation Research International Center (LIRIC) - U995
Kannagi, Reiji [Auteur]
Academia Sinica
Khoo, Kay-Hooi [Auteur]
National Taïwan University [NTU]
Yu, Shin [Auteur]

Journal title :

Glycobiology

Abbreviated title :

Glycobiology

Volume number :

28

Pages :

9-20

Publication date :

2018-01-01

Keyword(s) :

lymphocytes
glycomics
sulfoglycomics
PBMC
mass spectrometry

English keyword(s) :

glycomics
lymphocytes
mass spectrometry
PBMC
sulfoglycomics

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Despite well-recognized biological importance, mass spectrometry (MS)-based glycomic identification of sulfo-, sialylated terminal glyco-epitopes on the N-glycans of various immune cell types remains technically challenging ...
Show more >Despite well-recognized biological importance, mass spectrometry (MS)-based glycomic identification of sulfo-, sialylated terminal glyco-epitopes on the N-glycans of various immune cell types remains technically challenging and rarely reported. Previous studies with monoclonal antibody have implicated a regulated expression of 6-sulfo-α2-6-sialyl LacNAc on B cells in peripheral lymph nodes and the circulating peripheral blood lymphocytes but its occurrence on leukemia cells or lymphomas have not been critically addressed. In this study, we have extended our previously developed MS-based sulfoglycomic platform by incorporating additional complementary analytical approaches in order to achieve a high sensitivity mapping and relative quantification of the detected sulfated glycotopes down to the level of defining their sialyl linkages. We showed that discovery mode sulfoglycomics and precise location of sulfate were best achieved by multimode MS analyses of fractionated, permethylated sulfated N-glycans. On the other hand, the relative degree of sulfation on individual N-glycans could be more efficiently inferred from the respective extracted ion chromatograms of native, non-sulfated and sulfated target N-glycans in single LC–MS/MS runs. The GlcNAc-6-O-sulfated α2-6-sialyl LacNAc, which constitutes the higher affinity ligand for the human inhibitory co-receptor of B cells, CD22, was found to be commonly carried on a range of complex type N-glycans from human CD19+ and CD4+ lymphocytes. We further showed that its occurrence on the most abundant α2-6-disialylated biantennary structure from the peripheral blood mononuclear cells of patients diagnosed as B-cell chronic lymphocytic leukemia varied within ±2-fold abundance from the mean value determined for isolated CD19+ lymphocytes and cultured B-CLL cells.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Inserm
Université de Lille
CHU Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Lille Neurosciences & Cognition (LilNCog) - U 1172

Research team(s) :

Immunity, inflammation and fibrsis in auto and allo-reactivity

Submission date :

2019-03-01T14:34:54Z
2024-01-22T14:31:45Z