Influence of aging in the modulation of ...
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
Influence of aging in the modulation of epigenetic biomarkers of carcinogenesis after exposure to air pollution.
Author(s) :
Fougere, Bertrand [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
301404|||Université du Littoral Côte d'Opale [ULCO]
Landkocz, Yann [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Lepers, Capucine [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Martin Perrine, J [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Armand, Lucie [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Verdin, Anthony [Auteur]
Boulanger, Eric [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Gosset, Pierre [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Sichel, Francois [Auteur]
Shirali, Pirouz [Auteur]
Billet, Sylvain [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
301404|||Université du Littoral Côte d'Opale [ULCO]
Landkocz, Yann [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Lepers, Capucine [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Martin Perrine, J [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Armand, Lucie [Auteur]
Université du Littoral Côte d'Opale [ULCO]
Verdin, Anthony [Auteur]
Boulanger, Eric [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Gosset, Pierre [Auteur]
Groupement des Hôpitaux de l'Institut Catholique de Lille [GHICL]
Sichel, Francois [Auteur]
Shirali, Pirouz [Auteur]
Billet, Sylvain [Auteur]
Journal title :
Experimental Gerontology
Abbreviated title :
Exp. Gerontol.
Volume number :
110
Pages :
125-132
Publication date :
2018-09
ISSN :
1873-6815
Keyword(s) :
Biomarkers
PM2.5
PBMC
Epigenotoxicity
Aging
PM2.5
PBMC
Epigenotoxicity
Aging
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Background: Classified as carcinogenic to humans by the IARC in 2013, fine air particulate matter (PM2.5) can be inhaled and retained into the lung or reach the systemic circulation. This can cause or exacerbate numerous ...
Show more >Background: Classified as carcinogenic to humans by the IARC in 2013, fine air particulate matter (PM2.5) can be inhaled and retained into the lung or reach the systemic circulation. This can cause or exacerbate numerous pathologies to which the elderly are often more sensitive. Methods: In order to estimate the influence of age on the development of early cellular epigenetic alterations involved in carcinogenesis, peripheral blood mononuclear cells sampled from 90 patients from three age classes (25–30, 50–55 and 75–80 years old) were ex vivo exposed to urban PM2.5. Results: Particles exposure led to variations in telomerase activity and telomeres length in all age groups without any influence of age. Conversely, P16INK4A gene expression increased significantly with age after exposure to PM2.5. Age could enhance MGMT gene expression after exposure to particles, by decreasing the level of promoter methylation in the oldest people. Conclusion: Hence, our results demonstrated several tendencies in cells modification depending on age, even if all epigenetic assays were carried out after a limited exposure time allowing only one or two cell cycles. Since lung cancer symptoms appear only at an advanced stage, our results underline the needs for further investigation on the studied biomarkers for early diagnosis of carcinogenesis to improve survival.Show less >
Show more >Background: Classified as carcinogenic to humans by the IARC in 2013, fine air particulate matter (PM2.5) can be inhaled and retained into the lung or reach the systemic circulation. This can cause or exacerbate numerous pathologies to which the elderly are often more sensitive. Methods: In order to estimate the influence of age on the development of early cellular epigenetic alterations involved in carcinogenesis, peripheral blood mononuclear cells sampled from 90 patients from three age classes (25–30, 50–55 and 75–80 years old) were ex vivo exposed to urban PM2.5. Results: Particles exposure led to variations in telomerase activity and telomeres length in all age groups without any influence of age. Conversely, P16INK4A gene expression increased significantly with age after exposure to PM2.5. Age could enhance MGMT gene expression after exposure to particles, by decreasing the level of promoter methylation in the oldest people. Conclusion: Hence, our results demonstrated several tendencies in cells modification depending on age, even if all epigenetic assays were carried out after a limited exposure time allowing only one or two cell cycles. Since lung cancer symptoms appear only at an advanced stage, our results underline the needs for further investigation on the studied biomarkers for early diagnosis of carcinogenesis to improve survival.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Inserm
Université de Lille
CHU Lille
Université de Lille
CHU Lille
Research team(s) :
Glycation from inflammation to aging
Submission date :
2019-03-01T14:35:28Z
2021-06-09T12:49:28Z
2024-01-25T09:58:32Z
2021-06-09T12:49:28Z
2024-01-25T09:58:32Z