Probing nanoparticle-membrane interactions ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Titre :
Probing nanoparticle-membrane interactions by combining amphiphilic diblock copolymer assembly and plasmonics
Auteur(s) :
Koch, Amelie H. R. [Auteur]
Max Planck Institute for Polymer Research
Morsbach, Svenja [Auteur]
Max Planck Institute for Polymer Research
Bereau, Tristan [Auteur]
Max Planck Institute for Polymer Research
Leveque, Gaetan [Auteur]
Physique - IEMN [PHYSIQUE - IEMN]
Butt, Hans-Juergen [Auteur]
Max Planck Institute for Polymer Research
Deserno, Markus [Auteur]
Machine Learning Department [Carnegie Mellon Univ.]
Landfester, Katharina [Auteur]
Max Planck Institute for Polymer Research
Fytas, George [Auteur correspondant]
Max Planck Institute for Polymer Research
Max Planck Institute for Polymer Research
Morsbach, Svenja [Auteur]
Max Planck Institute for Polymer Research
Bereau, Tristan [Auteur]
Max Planck Institute for Polymer Research
Leveque, Gaetan [Auteur]
Physique - IEMN [PHYSIQUE - IEMN]
Butt, Hans-Juergen [Auteur]
Max Planck Institute for Polymer Research
Deserno, Markus [Auteur]
Machine Learning Department [Carnegie Mellon Univ.]
Landfester, Katharina [Auteur]
Max Planck Institute for Polymer Research
Fytas, George [Auteur correspondant]
Max Planck Institute for Polymer Research
Titre de la revue :
Journal of Physical Chemistry B
Pagination :
742-750
Éditeur :
American Chemical Society
Date de publication :
2020
ISSN :
1520-6106
Discipline(s) HAL :
Chimie/Polymères
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized ...
Lire la suite >Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized NPs, passive internalization is prompted by relatively unspecific parameters, such as particle size and charge. Based on theoretical treatments, adhesion to and bending of the cell membrane can induce NP wrapping. Experimentally, powerful tools are needed to selectively probe possible membrane-NP motifs at very dilute conditions and avoid dye labeling. In this work, we employ surface resonance-enhanced dynamic light scattering, surface plasmon resonance, electron microscopy, and simulations for sensing interactions between plasmonic AuNPs and polymersomes. We distinguish three different interaction scenarios at nanomolar concentrations by tuning the surface charge of AuNPs and rationalize these events by balancing vesicle bending and electrostatic/van der Waals AuNP and vesicle adhesion. The clarification of the physical conditions under which nanoparticles passively translocate across membranes can aid in the rational design of drugs that cannot exploit specific modes of cellular uptake and also elucidates physical properties that render nanoparticles in the environment particularly toxic.Lire moins >
Lire la suite >Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized NPs, passive internalization is prompted by relatively unspecific parameters, such as particle size and charge. Based on theoretical treatments, adhesion to and bending of the cell membrane can induce NP wrapping. Experimentally, powerful tools are needed to selectively probe possible membrane-NP motifs at very dilute conditions and avoid dye labeling. In this work, we employ surface resonance-enhanced dynamic light scattering, surface plasmon resonance, electron microscopy, and simulations for sensing interactions between plasmonic AuNPs and polymersomes. We distinguish three different interaction scenarios at nanomolar concentrations by tuning the surface charge of AuNPs and rationalize these events by balancing vesicle bending and electrostatic/van der Waals AuNP and vesicle adhesion. The clarification of the physical conditions under which nanoparticles passively translocate across membranes can aid in the rational design of drugs that cannot exploit specific modes of cellular uptake and also elucidates physical properties that render nanoparticles in the environment particularly toxic.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :
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