Simple Protocol to Purify Cell Wall ...
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity.
Author(s) :
Sadovskaya, Irina [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Institut Charles Viollette (ICV) - ULR 7394
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Journal title :
Methods in Molecular Biology (Clifton, N.J.)
Abbreviated title :
Methods Mol Biol
Volume number :
1954
Pages :
37-45
Publication date :
2019
ISSN :
1940-6029
English keyword(s) :
Acids
Cell Wall
Chemical Fractionation
Chromatography, Gel
Gram-Positive Bacteria
Gram-Positive Bacterial Infections
Humans
Magnetic Resonance Spectroscopy
Polysaccharides, Bacterial
Cell wall polysaccharide
HR-MAS NMR
Purification
Cell Wall
Chemical Fractionation
Chromatography, Gel
Gram-Positive Bacteria
Gram-Positive Bacterial Infections
Humans
Magnetic Resonance Spectroscopy
Polysaccharides, Bacterial
Cell wall polysaccharide
HR-MAS NMR
Purification
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Cell wall polysaccharides (CWPS), which are usually covalently bound to the peptidoglycan and are closely associated with the cell wall, are considered as ubiquitous components of the cell envelope of gram-positive bacteria ...
Show more >Cell wall polysaccharides (CWPS), which are usually covalently bound to the peptidoglycan and are closely associated with the cell wall, are considered as ubiquitous components of the cell envelope of gram-positive bacteria and play an important role as mediators of bacterial interactions with the environment. Here, we describe a simple method for purifying CWPS by extraction of bacterial cells with consecutive acid treatments. Purified CWPS are obtained by gel-filtration chromatography following treatment with HF. We also provide the methodology to easily assess the integrity of CWPS using high-resolution magic-angle spinning (HR-MAS) NMR.Show less >
Show more >Cell wall polysaccharides (CWPS), which are usually covalently bound to the peptidoglycan and are closely associated with the cell wall, are considered as ubiquitous components of the cell envelope of gram-positive bacteria and play an important role as mediators of bacterial interactions with the environment. Here, we describe a simple method for purifying CWPS by extraction of bacterial cells with consecutive acid treatments. Purified CWPS are obtained by gel-filtration chromatography following treatment with HF. We also provide the methodology to easily assess the integrity of CWPS using high-resolution magic-angle spinning (HR-MAS) NMR.Show less >
Language :
Anglais
Peer reviewed article :
Non
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Chemical Glycobiology
Submission date :
2021-10-18T12:54:07Z
2021-10-19T16:41:45Z
2021-10-19T16:41:45Z
Files
- P19.30 Simple Protocol to Purify.pdf
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