Titre :

Simple Protocol to Purify Cell Wall Polysaccharide from Gram-Positive Bacteria and Assess Its Structural Integrity.

Auteur(s) :

Sadovskaya, Irina [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Titre de la revue :

Methods in Molecular Biology (Clifton, N.J.)

Nom court de la revue :

Methods Mol Biol

Numéro :

1954

Pagination :

37-45

Date de publication :

2019

ISSN :

1940-6029

Mot(s)-clé(s) en anglais :

Acids
Cell Wall
Chemical Fractionation
Chromatography, Gel
Gram-Positive Bacteria
Gram-Positive Bacterial Infections
Humans
Magnetic Resonance Spectroscopy
Polysaccharides, Bacterial
Cell wall polysaccharide
HR-MAS NMR
Purification

Discipline(s) HAL :

Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

Cell wall polysaccharides (CWPS), which are usually covalently bound to the peptidoglycan and are closely associated with the cell wall, are considered as ubiquitous components of the cell envelope of gram-positive bacteria ...
Lire la suite >Cell wall polysaccharides (CWPS), which are usually covalently bound to the peptidoglycan and are closely associated with the cell wall, are considered as ubiquitous components of the cell envelope of gram-positive bacteria and play an important role as mediators of bacterial interactions with the environment. Here, we describe a simple method for purifying CWPS by extraction of bacterial cells with consecutive acid treatments. Purified CWPS are obtained by gel-filtration chromatography following treatment with HF. We also provide the methodology to easily assess the integrity of CWPS using high-resolution magic-angle spinning (HR-MAS) NMR.Lire moins >

Langue :

Anglais

Comité de lecture :

Non

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Chemical Glycobiology

Date de dépôt :

2021-10-18T12:54:07Z
2021-10-19T16:41:45Z