Title :

Immunomagnetic selective donor-derived CD4+CCR7+ T cell depletion procedure for peripheral blood stem cells graft

Author(s) :

Varlet, Pauline [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Rogeau, Stéphanie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Trauet, Jacques [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Demaret, Julie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Labalette, Myriam [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

Current Research in Translational Medicine

Abbreviated title :

Current Research in Translational Medicine

Volume number :

67

Pages :

1-7

Publication date :

2019-02

ISSN :

24523186

English abstract : [en]

Purpose of the study: While acute graft-versus-host-disease (GVHD) is a T cell-mediated disease caused by alloreactive donor T cells, we and others have highlighted that patients who received higher proportion of donor ...
Show more >Purpose of the study: While acute graft-versus-host-disease (GVHD) is a T cell-mediated disease caused by alloreactive donor T cells, we and others have highlighted that patients who received higher proportion of donor CD4+ naïve and central memory T cells expressing the chemokine receptor 7 (CCR7) more often developed acute GVHD than those who did not. Consequently, we then investigated in vitro the impact of selective CD4+ CCR7+ T cell depletion on immune reactions and showed that such a depletion reduced alloreactivity without altering acquired anti-infectious reactions. In order to translate these findings to clinic, we now developed a compliant procedure for a selective reduction of the CD4+ naïve and central memory T cell subset relevant to peripheral blood stem cell (PBSC) allografts. Patients and methods: We performed a two-step immunomagnetic depletion of CD4+ CCR7+ T cells from ten G-CSF-mobilized PBSC apheresis samples. Results: A median of 89% (82–94%) of CD4+ CCR7+ T cells could be depleted. This allowed a marked reduction of the alloreactive immune response against allogenic dendritic cells compared with unmanipulated cells. The preservation of CD34+ cell number and the hematopoietic progenitor function were controlled. Functional tests showed that the selection procedure did not interfere with the capacity of pathogen-specific T cells to produce interferon-gamma in response to certain viral pathogens. Conclusion: Our results pave the way to a feasible procedure that can be used in patients undergoing allo-hematopoietic cell transplantation and particularly for improving haploidentical transplant results by controlling GVHD, the main immune complication.Show less >

Language :

Anglais

Audience :

Non spécifiée

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2021-12-08T09:52:45Z
2024-02-02T09:31:20Z