Title :

Proteolytic activity of Lactobacillus strains isolated from Mongolian traditional dairy products: A multiparametric analysis

Author(s) :

Raveschot, Cyril [Auteur]
Institut Charles Viollette (ICV) - ULR 7394 [ICV]
Cudennec, Benoit [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Deracinois, Barbara [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Frémont, Marc [Auteur]
Vaeremans, Maxime [Auteur]
Dugersuren, Jamyan [Auteur]
Demberel, Shirchin [Auteur]
Drider, Djamel [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Dhulster, Pascal [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Coutte, Francois [Auteur]
Institut Charles Viollette (ICV) - ULR 7394 [ICV]
Flahaut, Christophe [Auteur]
Institut Charles Viollette (ICV) - ULR 7394 [ICV]

Journal title :

Food Chemistry

Volume number :

304

Pages :

125415

Publisher :

Elsevier

Publication date :

2020-01-30

ISSN :

0308-8146

English keyword(s) :

Cell envelope associated peptidases
fermented milk
Lactobacillus
peptides

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The aim of our study was to characterize the proteolytic activity of 170 Lactobacillus strains isolated from traditional Mongolian dairy products (yogurt and fermented milk), and to investigate their capacity to generate ...
Show more >The aim of our study was to characterize the proteolytic activity of 170 Lactobacillus strains isolated from traditional Mongolian dairy products (yogurt and fermented milk), and to investigate their capacity to generate bioactive peptides during milk fermentation. All isolates were screened for proteolytic activity using skim milk agar-well diffusion test. Fifteen strains (9 Lactobacillus helveticus and 6 Lactobacillus delbrueckii subsp. bulgaricus) were then selected and further evaluated using an original strategy based on multiparametric analysis, taking into account growth rate, acidification capacity, proteolytic activity, cell envelope associated peptidase (CEP) profile and LC–MS/MS analysis of peptides. All parameters were analyzed using principal component analysis (PCA). Results showed that strain growth and acidification correlate with peptide production and that Mongolian L. helveticus strains differ from Western strains in terms of CEP distribution. The PCA revealed that CEP profiles are major determinants of β-casein hydrolysis patterns. Strains with distinctive proteolytic activities were identified.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Plateforme intégREe AppLiquée au criblage haut débit de CATalyseurs pour les bioraffineries

Administrative institution(s) :

INRA
ISA
Univ. Artois
Univ. Littoral Côte d’Opale
Université de Lille

Collections :

• BioEcoAgro - UMR-T 1158

Submission date :

2021-12-08T09:53:24Z
2024-04-29T14:49:52Z