Title :

Metal enriched quasi-ultrafine particles from stainless steel gas metal arc welding induced genetic and epigenetic alterations in beas-2b cells

Author(s) :

Boudjema, Jamila [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
171461|||Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS] (VALID)
Lima, B. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Grare, Celine [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Alleman, L. Y. [Auteur]
Ecole nationale supérieure Mines-Télécom Lille Douai [IMT Nord Europe]
Rousset, D. [Auteur]
Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) [INRS ( Vandoeuvre lès Nancy)]
Perdrix, Esperanza [Auteur]
Ecole nationale supérieure Mines-Télécom Lille Douai [IMT Nord Europe]
Achour, Djamal [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Anthérieu, Sébastien [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Platel, Anne [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Nesslany, Fabrice [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Leroyer, Ariane [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Nisse, C. [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Lo-Guidice, Jean-Marc [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Garçon, Guillaume [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Journal title :

NanoImpact

Abbreviated title :

NanoImpact

Volume number :

23

Publication date :

2021-07-01

ISSN :

2452-0748

Keyword(s) :

Oxidative stress
Intrinsic oxidative potential
Human bronchial epithelial BEAS-2B cells
Quasi-ultrafine particles
Metals
Welding fume
Genetic and epigenetic alterations

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Recent evidence has supported welding fume (WF)-derived ultrafine particles (UFP) could be the driving force of their adverse health effects. However, UFP have not yet been extensively studied and are currently not included ...
Show more >Recent evidence has supported welding fume (WF)-derived ultrafine particles (UFP) could be the driving force of their adverse health effects. However, UFP have not yet been extensively studied and are currently not included in present air quality standards/guidelines. Here, attention was focused on the underlying genetic and epigenetic mechanisms by which the quasi-UFP (Q-UFP, i.e., ≤ 0.25 μm) of the WF emitted by gas metal arc welding-stainless steel (GMAW-SS) exert their toxicity in human bronchial epithelial BEAS-2B cells. The Q-UFP under study showed a monomodal size distribution in number centered on 104.4 ± 52.3 nm and a zeta potential of −13.8 ± 0.3 mV. They were enriched in Fe > Cr > Mn > Si, and displayed a relatively high intrinsic oxidative potential. Dose-dependent activation of nuclear factor erythroid 2-related factor 2 and nuclear factor-kappa B signaling pathway, glutathione alteration, and DNA, protein and lipid oxidative damage were reported in BEAS-2B cells acutely (1.5 and 9 μg/cm2, 24 h) or repeatedly (0.25 and 1.5 μg/cm2, 3 × 24 h) exposed to Q-UFP (p < 0.05). Alterations of the Histone H3 acetylation were reported for any exposure (p < 0.05). Differentially regulated miRNA and mRNA indicated the activation of some critical cell signaling pathways related to oxidative stress, inflammation, and cell cycle deregulation towards apoptosis. Taken together, these results highlighted the urgent need to better evaluate the respective toxicity of the different metals and to include the Q-UFP fraction of WF in current air quality standards/guidelines relevant to the occupational settings.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Institut Pasteur de Lille
Université de Lille

Collections :

• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2022-02-02T10:25:02Z
2024-02-05T13:55:29Z
2024-02-23T10:27:14Z