Title :

Evolution of the scattering properties of phytoplankton cells from flow cytometry measurements

Author(s) :

Moutier, William [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Duforêt-Gaurier, Lucile [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Thyssen, Melilotus [Auteur]
Institut méditerranéen d'océanologie [MIO]
Loisel, Hubert [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Meriaux, Xavier [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Courcot, Lucie [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Dessailly, David [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Rêve, Anne-Hélène [Auteur]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Grégori, Gérald [Auteur]
Institut méditerranéen d'océanologie [MIO]
Alvain, Séverine [Auteur]
Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] [LSCE]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Barani, Aude [Auteur]
Institut méditerranéen d'océanologie [MIO]
Brutier, Laurent [Auteur]
Laboratoire Ecologie, Evolution, Interactions des Systèmes amazoniens [LEEISA]
Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 [LOG]
Université du Littoral Côte d'Opale [ULCO]
Dugenne, Mathilde [Auteur]
Institut méditerranéen d'océanologie [MIO]

Journal title :

PLoS One

Pages :

1-24 / e0181180

Publisher :

Public Library of Science

Publication date :

2017-07-14

ISSN :

1932-6203

HAL domain(s) :

Sciences du Vivant [q-bio]/Ecologie, Environnement

English abstract : [en]

After the exponential growth phase, variability in the scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent cells and detritrus. ...
Show more >After the exponential growth phase, variability in the scattering efficiency of phytoplankton cells over their complete life cycle is not well characterised. Bulk measurements are impacted by senescent cells and detritrus. Thus the analysis of the evolution of the optical properties thanks to their morphological and/or intra-cellular variations remains poorly studied. Using the Cytosense flow cytometer (CytoBuoy b.v., NL), the temporal course of the forward and sideward efficiencies of two phytoplankton species (Thalassiosira pseudonana and Chlamydomonas concordia) were analyzed during a complete life-cycle. These two species differ considerably from a morphological point of view. Over the whole experiment, the forward and sideward efficiencies of Thalassiosira pseudonana were, on average, respectively 2.2 and 1.6 times higher than the efficiencies of Chlamydomonas concordia. Large intra-species variability of the efficiencies were observed over the life cycle of the considered species. It highlights the importance of considering the optical properties of phytoplankton cells as a function of the population growth stage of the considered species. Furthermore, flow cytometry measurements were combined with radiative transfer simulations and biogeochemical and optical measurements. Results showed that the real refractive index of the chloroplast is a key parameter driving the sideward signal and that a simplistic two-layered model (cytoplasm-chloroplast) seems particularly appropriate to represent the phytoplankton cells.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Laboratoire d'Océanologie et de Géosciences (LOG) - UMR 8187

Source :

Harvested from HAL