Bioid screen of salmonella type 3 secreted ...
Type de document :
Article dans une revue scientifique: Article original
PMID :
URL permanente :
Titre :
Bioid screen of salmonella type 3 secreted effectors reveals host factors involved in vacuole positioning and stability during infection
Auteur(s) :
D'costa, Vanessa M. [Auteur]
Coyaud, Etienne-Marie [Auteur]
Boddy, Kirsten C. [Auteur]
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
St-Germain, Jonathan R. [Auteur]
Li, Taoyingnan [Auteur]
Grinstein, Sergio [Auteur]
Raught, Brian [Auteur]
Brumell, John H. [Auteur]
Coyaud, Etienne-Marie [Auteur]
Boddy, Kirsten C. [Auteur]
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
St-Germain, Jonathan R. [Auteur]
Li, Taoyingnan [Auteur]
Grinstein, Sergio [Auteur]
Raught, Brian [Auteur]
Brumell, John H. [Auteur]
Titre de la revue :
Nature microbiology
Nom court de la revue :
NAT. MICROBIOL
Numéro :
4
Pagination :
-
Date de publication :
2019-12-01
ISSN :
2058-5276
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Many bacterial pathogens express virulence proteins that are translocated into host cells (herein referred to as effectors), where they can interact with target proteins to manipulate host cell processes. These effector-host ...
Lire la suite >Many bacterial pathogens express virulence proteins that are translocated into host cells (herein referred to as effectors), where they can interact with target proteins to manipulate host cell processes. These effector-host protein interactions are often dynamic and transient in nature, making them difficult to identify using traditional interaction-based methods. Here, we performed a systematic comparison between proximity-dependent biotin labelling (BioID) and immunoprecipitation coupled with mass spectrometry to investigate a series of Salmonella type 3 secreted effectors that manipulate host intracellular trafficking (SifA, PipB2, SseF, SseG and SopD2). Using BioID, we identified 632 candidate interactions with 381 unique human proteins, collectively enriched for roles in vesicular trafficking, cytoskeleton components and transport activities. From the subset of proteins exclusively identified by BioID, we report that SifA interacts with BLOC-2, a protein complex that regulates dynein motor activity. We demonstrate that the BLOC-2 complex is necessary for SifA-mediated positioning of Salmonella-containing vacuoles, and affects stability of the vacuoles during infection. Our study provides insight into the coordinated activities of Salmonella type 3 secreted effectors and demonstrates the utility of BioID as a powerful, complementary tool to characterize effector-host protein interactions.Lire moins >
Lire la suite >Many bacterial pathogens express virulence proteins that are translocated into host cells (herein referred to as effectors), where they can interact with target proteins to manipulate host cell processes. These effector-host protein interactions are often dynamic and transient in nature, making them difficult to identify using traditional interaction-based methods. Here, we performed a systematic comparison between proximity-dependent biotin labelling (BioID) and immunoprecipitation coupled with mass spectrometry to investigate a series of Salmonella type 3 secreted effectors that manipulate host intracellular trafficking (SifA, PipB2, SseF, SseG and SopD2). Using BioID, we identified 632 candidate interactions with 381 unique human proteins, collectively enriched for roles in vesicular trafficking, cytoskeleton components and transport activities. From the subset of proteins exclusively identified by BioID, we report that SifA interacts with BLOC-2, a protein complex that regulates dynein motor activity. We demonstrate that the BLOC-2 complex is necessary for SifA-mediated positioning of Salmonella-containing vacuoles, and affects stability of the vacuoles during infection. Our study provides insight into the coordinated activities of Salmonella type 3 secreted effectors and demonstrates the utility of BioID as a powerful, complementary tool to characterize effector-host protein interactions.Lire moins >
Langue :
Anglais
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
INSERM
Université de Lille
Université de Lille
Date de dépôt :
2022-06-15T13:57:47Z