Comparative super-resolution mapping of ...
Document type :
Article dans une revue scientifique: Article original
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Title :
Comparative super-resolution mapping of basal feet reveals a modular but distinct architecture in primary and motile cilia
Author(s) :
Nguyen, Quynh P. H. [Auteur]
University of Toronto
Liu, Zhen [Auteur]
University of Toronto
Albulescu, Alexandra [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Ouyang, Hong [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Zlock, Lorna [Auteur]
California Northstate University [CNSU]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Finkbeiner, Walter [Auteur]
California Northstate University [CNSU]
Moraes, Theo J. [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Raught, Brian [Auteur]
University of Toronto
Mennella, Vito [Auteur]
University of Toronto
University of Toronto
Liu, Zhen [Auteur]
University of Toronto
Albulescu, Alexandra [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Ouyang, Hong [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Zlock, Lorna [Auteur]
California Northstate University [CNSU]
Coyaud, Etienne-Marie [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Laurent, Estelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Finkbeiner, Walter [Auteur]
California Northstate University [CNSU]
Moraes, Theo J. [Auteur]
The Hospital for sick children [Toronto] [SickKids]
Raught, Brian [Auteur]
University of Toronto
Mennella, Vito [Auteur]
University of Toronto
Journal title :
Developmental cell
Abbreviated title :
Dev. Cell
Volume number :
55
Pages :
209-223.E7
Publisher :
Elsevier
Publication date :
2020-10-26
ISSN :
1534-5807
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. ...
Show more >In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. Through a super-resolution-driven approach, here we characterize the organization of the ciliary basal foot, an appendage of basal bodies whose main role is to provide a point of anchoring to the microtubule cytoskeleton. Quantitative image analysis shows that the basal foot is organized into three main regions linked by elongated coiled-coil proteins, revealing a conserved modular architecture in primary and motile cilia, but showing distinct features reflecting its specialized functions. Using domain-specific BioID proximity labeling and super-resolution imaging, we identify CEP112 as a basal foot protein and other candidate components of this assembly, aiding future investigations on the role of basal foot across different cilia systems.Show less >
Show more >In situ molecular architecture analysis of organelles and protein assemblies is essential to understanding the role of individual components and their cellular function, and to engineering new molecular functionalities. Through a super-resolution-driven approach, here we characterize the organization of the ciliary basal foot, an appendage of basal bodies whose main role is to provide a point of anchoring to the microtubule cytoskeleton. Quantitative image analysis shows that the basal foot is organized into three main regions linked by elongated coiled-coil proteins, revealing a conserved modular architecture in primary and motile cilia, but showing distinct features reflecting its specialized functions. Using domain-specific BioID proximity labeling and super-resolution imaging, we identify CEP112 as a basal foot protein and other candidate components of this assembly, aiding future investigations on the role of basal foot across different cilia systems.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
INSERM
Université de Lille
Université de Lille
Submission date :
2022-06-15T13:58:01Z
2023-03-08T08:30:10Z
2024-02-14T07:18:33Z
2024-05-22T08:41:28Z
2023-03-08T08:30:10Z
2024-02-14T07:18:33Z
2024-05-22T08:41:28Z
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