Carbonic anhydrase xii expression is ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
Permalink :
Title :
Carbonic anhydrase xii expression is modulated during epithelial mesenchymal transition and regulated through protein kinase c signaling
Author(s) :
Vergara, Daniele [Auteur]
University of Salento [Lecce]
Ravaioli, Sara [Auteur]
Unit of Melanoma Medical Oncology [Fondazione IRCCS Istituto Nazionale Tumori, Milan]
Fonzi, Eugenio [Auteur]
Adamo, Loredaria [Auteur]
Damato, Marina [Auteur]
Bravaccini, Sara [Auteur]
Pirini, Francesca [Auteur]
Gaballo, Antonio [Auteur]
Barbano, Raffaela [Auteur]
Pasculli, Barbara [Auteur]
Franck, Julien [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
FOURNIER, Isabelle [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Maffia, Michele [Auteur]
University of Salento [Lecce]
Ravaioli, Sara [Auteur]
Unit of Melanoma Medical Oncology [Fondazione IRCCS Istituto Nazionale Tumori, Milan]
Fonzi, Eugenio [Auteur]
Adamo, Loredaria [Auteur]
Damato, Marina [Auteur]
Bravaccini, Sara [Auteur]
Pirini, Francesca [Auteur]
Gaballo, Antonio [Auteur]
Barbano, Raffaela [Auteur]
Pasculli, Barbara [Auteur]
Franck, Julien [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
FOURNIER, Isabelle [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Salzet, Michel [Auteur]
![refId](/themes/Mirage2//images/idref.png)
Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192
Maffia, Michele [Auteur]
Journal title :
International Journal of Molecular Sciences
Abbreviated title :
Int J Mol Sci
Volume number :
21
Pages :
715
Publication date :
2020-01-22
ISSN :
1422-0067
Keyword(s) :
breast cancer
carbonic anhydrase
epithelial mesenchymal transition
proteomics
PKC
transport metabolon
metabolism
carbonic anhydrase
epithelial mesenchymal transition
proteomics
PKC
transport metabolon
metabolism
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Members of the carbonic anhydrase family are functionally involved in the regulation of intracellular and extracellular pH in physiological and pathological conditions. Their expression is finely regulated to maintain a ...
Show more >Members of the carbonic anhydrase family are functionally involved in the regulation of intracellular and extracellular pH in physiological and pathological conditions. Their expression is finely regulated to maintain a strict control on cellular homeostasis, and it is dependent on the activation of extracellular and intracellular signaling pathways. Combining RNA sequencing (RNA-seq), NanoString, and bioinformatics data, we demonstrated that the expression of carbonic anhydrase 12 (CAXII) is significantly different in luminal and triple negative breast cancer (BC) models and patients, and is associated with the activation of an epithelial mesenchymal transition (EMT) program. In BC models, the phorbol ester 12-myristate 13-acetate (PMA)-mediated activation of protein kinase C (PKC) induced a down-regulation of CAXII with a concomitant modulation of other members of the transport metabolon, including CAIX and the sodium bicarbonate cotransporter 3 (NBCn1). This is associated with a remodeling of tumor glycolytic metabolism induced after PKC activation. Overall, this analysis highlights the dynamic nature of transport metabolom and identifies signaling pathways finely regulating this plasticity.Show less >
Show more >Members of the carbonic anhydrase family are functionally involved in the regulation of intracellular and extracellular pH in physiological and pathological conditions. Their expression is finely regulated to maintain a strict control on cellular homeostasis, and it is dependent on the activation of extracellular and intracellular signaling pathways. Combining RNA sequencing (RNA-seq), NanoString, and bioinformatics data, we demonstrated that the expression of carbonic anhydrase 12 (CAXII) is significantly different in luminal and triple negative breast cancer (BC) models and patients, and is associated with the activation of an epithelial mesenchymal transition (EMT) program. In BC models, the phorbol ester 12-myristate 13-acetate (PMA)-mediated activation of protein kinase C (PKC) induced a down-regulation of CAXII with a concomitant modulation of other members of the transport metabolon, including CAIX and the sodium bicarbonate cotransporter 3 (NBCn1). This is associated with a remodeling of tumor glycolytic metabolism induced after PKC activation. Overall, this analysis highlights the dynamic nature of transport metabolom and identifies signaling pathways finely regulating this plasticity.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
INSERM
Université de Lille
Université de Lille
Submission date :
2022-06-15T13:59:25Z
2023-01-11T11:14:40Z
2023-01-11T11:14:40Z
Files
- ijms-21-00715-v2.pdf
- Version éditeur
- Open access
- Access the document