Titre :

Effect of calcium on the thermal denaturation of whey proteins and subsequent fouling in a benchtop fouling device: An experimental and numerical approach

Auteur(s) :

Liu, Weiji [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Feng, Yuting [Auteur]
Pan, Fei [Auteur]
Jeantet, Romain [Auteur]
1096408|||Science et Technologie du Lait et de l'Oeuf [STLO]
Andre, Christophe [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Chen, Xiao Dong [Auteur]
FoodPRINT International Associated Laboratory INRAE, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Materials Science
Delaplace, Guillaume [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207

Titre de la revue :

FOOD AND BIOPRODUCTS PROCESSING

Numéro :

136

Éditeur :

Elsevier

Date de publication :

2022-09-13

ISSN :

0960-3085

Mot(s)-clé(s) en anglais :

Whey protein fouling approche
Ionic calcium
Benchtop fouling device
3D CFD simulation
Temperature quantification
Pyrromethene 556

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Ingénierie des aliments
Sciences de l'ingénieur [physics]/Génie des procédés

Résumé en anglais : [en]

The key role of calcium in whey protein fouling behavior is well known in plate heat exchangers (PHEs), as it affects both the thermal denaturation of proteins and deposition reaction on the surface. However, the complex ...
Lire la suite >The key role of calcium in whey protein fouling behavior is well known in plate heat exchangers (PHEs), as it affects both the thermal denaturation of proteins and deposition reaction on the surface. However, the complex flow pattern and the closed configuration of PHEs make it hard to investigate this phenomenon in situ. In this paper, a microchannel benchtop fouling device was designed, making it possible to achieve a similar temperature profile to that performed in PHEs but in a laminar regime. A 3D simulation was developed to predict the thermal denaturation of β-lactoglobulin (BLG) as well as its deposition in the microchannel using computational fluid dynamics (CFD). The thermodynamics and heat transfer of the numerical model was validated by experimentally measuring the bulk fluid temperature profile in the microchannel using fluorescence microscopy, where Pyrromethene 556 was used as a temperature indicator. Results revealed a quasi-linear relationship between the pre-exponential factor of deposition reaction and the calcium concentration, implying that the fouling was built in such a pattern that only one calcium ion per one BLG molecule is involved. The imaging of the fouling deposit in situ showed spherical structures of deposits at low calcium levels, while denser and more rod-like ones were found at higher calcium concentrations. The fouling behavior was found to follow a crystallization-like pattern with preference upon the previously fouled layer instead of clean stainless steel surface. These findings confirm the essential role of ionic calcium on the formation of fouling deposits by anchoring the denatured BLG protein upon the surface as well as strengthening the protein-protein interactions for fouling build-up.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS
INRAE
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Équipe(s) de recherche :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Date de dépôt :

2022-12-01T09:00:58Z