Title :

A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment

Author(s) :

Ladaigue, Ségolène [Auteur]
Lefranc, Anne-Charlotte [Auteur]
Balde, Khadidiatou [Auteur]
Quitoco, Monica [Auteur]
Bacquer, Emilie [Auteur]
Busso, Didier [Auteur]
Piton, Guillaume [Auteur]
Dépagne, Jordane [Auteur]
Déchamps, Nathalie [Auteur]
Yamakawa, Nao [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Debusschere, Louise [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Han, Chunxue [Auteur]
Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 [PLBS]
Allain, Fabrice [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Buard, Valérie [Auteur]
Tarlet, Georges [Auteur]
François, Agnès [Auteur]
Paget, Vincent [Auteur]
Milliat, Fabien [Auteur]
Guipaud, Olivier [Auteur]

Journal title :

iScience

Abbreviated title :

iScience

Volume number :

25

Pages :

105482

Publisher :

Elsevier BV

Publication date :

2022-12-22

ISSN :

2589-0042

HAL domain(s) :

Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique

English abstract : [en]

Radiation therapy damages tumors and normal tissues, probably in part through the recruitment of immune cells. Endothelial high-mannose N-glycans are, in particular, involved in monocyte-endothelium interactions. Trimmed ...
Show more >Radiation therapy damages tumors and normal tissues, probably in part through the recruitment of immune cells. Endothelial high-mannose N-glycans are, in particular, involved in monocyte-endothelium interactions. Trimmed by the class I α-mannosidases, these structures are quite rare in normal conditions. Here, we show that the expression of the endothelial α-mannosidase MAN1C1 protein decreases after irradiation. We modeled two crucial steps in monocyte recruitment by developing in vitro real-time imaging models. Inhibition of MAN1C1 expression by siRNA gene silencing increases the abundance of high-mannose N-glycans, improves the adhesion of monocytes on endothelial cells in flow conditions and, in contrast, decreases radiation-induced transendothelial migration of monocytes. Consistently, overexpression of MAN1C1 in endothelial cells using lentiviral vectors decreases the abundance of high-mannose N-glycans and monocyte adhesion and enhances transendothelial migration of monocytes. Hence, we propose a role for endothelial MAN1C1 in the recruitment of monocytes, particularly in the adhesion step to the endothelium.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Diversité structurale des héparanes sulfates et régulation de la réponse inflammatoire

Research platform(s) :

Plateforme d’Analyse des Glycoconjugués (PAGéS)

Submission date :

2023-01-06T10:06:01Z
2023-01-06T15:53:42Z