Title :

Parietal composition of Lichtheimia corymbifera: differences between spore and germ tube stages and host-pathogen interactions

Author(s) :

Lecointe, Karine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Coulon, Pauline [Auteur]
Krzewinski, Frederic [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Charlet, Rogatien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bortolus, Clovis [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Cornu, Marjorie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Coulon, Pauline [Auteur]

Journal title :

Medical Mycology

Publisher :

Oxford University Press (OUP)

Publication date :

2022-12-24

ISSN :

1369-3786

English keyword(s) :

Mucorales
cell wall
polysaccharides
Lichtheimia corymbifera

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

The molecular composition and structural organisation of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens. Although the organisation of the fungal cell wall, composed of 90% ...
Show more >The molecular composition and structural organisation of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens. Although the organisation of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors. Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy. This study also characterises the response of THP-1 cells to Mucorales. The study identified the presence of uncommon monosaccharides (fucose, galactose and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganisation. Immunofluorescence studies confirmed the exposure of β-glucan on the surface of swollen spores and germ-tubes. Both spores and germ-tubes of L. corymbifera promoted an early and strong pro-inflammatory response, through TLR-2. Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiology in fungal Pathogenesis and Clinical Applications
Chemical Glycobiology

Submission date :

2023-01-11T11:15:17Z
2023-01-13T08:58:02Z
2023-02-27T16:21:18Z