Title :

Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?

Author(s) :

Raulo, Roxane [Auteur]
Heuson, Egon [Auteur]
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Froidevaux, Rénato [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158
Phalip, Vincent [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
BioEcoAgro - UMR transfrontalière INRAe - UMRT1158

Journal title :

Biotechnology Letters

Abbreviated title :

Biotechnol Lett

Volume number :

43

Pages :

2283–2298

Publisher :

Springer

Publication date :

2021-10-27

ISSN :

1573-6776

English keyword(s) :

Saccharification
Lignin
Fungal enzymatic cocktails
Lignocellulosic biomass
Enzyme

HAL domain(s) :

Sciences du Vivant [q-bio]/Biotechnologies

English abstract : [en]

Purpose In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of ...
Show more >Purpose In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails. Methods Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities. Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates). The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails. Results The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails. On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient. However, the results on CBS substrates were more contrasted depending on the biomass tested. Altogether, the results highlighted that assessing laccase activities and taking into account the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails. Conclusion The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation. The saccharification assay proved to be essential to validate the data obtained from both simple and complex substrates.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
Centrale Lille
ENSCL
Univ. Artois

Collections :

• BioEcoAgro - UMR-T 1158

Submission date :

2023-05-30T15:27:51Z
2023-06-22T17:33:33Z