Title :

Synthesis and Complexation Ability of a Novel Bis-(guanidinium)-tetrakis-(beta-cyclodextrin) Dendrimeric Tetrapod as a Potential Gene Delivery (DNA and siRNA) System. Study of Cellular siRNA Transfection

Author(s) :

Menuel, Stephane [Auteur]
UCCS Équipe Catalyse Supramoléculaire
Fontanay, S. [Auteur]
Clarot, I. [Auteur]
Duval, R. E. [Auteur]
Diez, L. [Auteur]
Marsura, A. [Auteur]

Journal title :

Bioconjugate Chem.

Abbreviated title :

Bioconjugate Chem.

Pages :

2357-2362

Publication date :

2008-11-18

ISSN :

1043-1802

HAL domain(s) :

Chimie

English abstract : [en]

The facile synthesis of a novel bis-(guanidinium)-tetrakis-(β-cyclodextrin) tetrapod, the first example of a new host family, was described, and the ability of the cyclodextrin CyD tetrapod to form molecular association ...
Show more >The facile synthesis of a novel bis-(guanidinium)-tetrakis-(β-cyclodextrin) tetrapod, the first example of a new host family, was described, and the ability of the cyclodextrin CyD tetrapod to form molecular association with siRNA and DNA guest molecules was demonstrated. Affinity capillary electrophoresis was used to determine the binding constant with the evaluation of the shift in the electrophoretic mobility μ of injected siRNA when various CyD tetrapod concentrations were added to the run buffer. A significant association constant (Ka =16 000 M−1) was obtained with borate buffer when double-stranded siRNA was primarily opened with the help of temperature. An efficient cellular transfection of siRNA into human embryonic lung fibroblasts was observed by fluorescence microscopy.Show less >

Language :

Anglais

Administrative institution(s) :

Université de Lille
CNRS
Centrale Lille
ENSCL
Univ. Artois

Collections :

• Unité de Catalyse et Chimie du Solide (UCCS) - UMR 8181

Research team(s) :

Catalyse et chimie supramoléculaire (CASU)

Submission date :

2023-05-30T18:09:56Z