Title :

Role Played by Exosporium Glycoproteins in the Surface Properties of Bacillus cereus Spores and in Their Adhesion to Stainless Steel

Author(s) :

Lequette, Yannick [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Garenaux, Estelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Tauveron, Grégoire [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Dumez, Sylvain [Auteur]
Laboratoire de Génie des Procédés et Technologie Alimentaires [LGPTA]
Perchat, Stéphane [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Slomianny, Christian [Auteur]
Laboratoire de Physiologie Cellulaire - U 1003 [PHYCELL]
Lereclus, Didier [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]

Journal title :

Applied and Environmental Microbiology

Volume number :

77

Pages :

4905-4911

Publication date :

2011-07

HAL domain(s) :

Sciences du Vivant [q-bio]/Ingénierie des aliments
Physique [physics]/Matière Condensée [cond-mat]/Science des matériaux [cond-mat.mtrl-sci]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
Sciences de l'ingénieur [physics]/Génie des procédés

English abstract : [en]

Bacillus cereus spores are surrounded by a loose-fitting layer called the exosporium, whose distal part is mainly formed from glycoproteins. The role played by the exosporium glycoproteins of B. cereus ATCC 14579 (BclA and ...
Show more >Bacillus cereus spores are surrounded by a loose-fitting layer called the exosporium, whose distal part is mainly formed from glycoproteins. The role played by the exosporium glycoproteins of B. cereus ATCC 14579 (BclA and ExsH) was investigated by considering hydrophobicity and charge, as well as the properties of spore adhesion to stainless steel. The absence of BclA increased both the isoelectric point (IEP) and hydrophobicity of whole spores while simultaneously reducing the interaction between spores and stainless steel. However, neither the hydrophobicity nor the charge associated with BclA could explain the differences in the adhesion properties. Conversely, ExsH, another exosporium glycoprotein, did not play a significant role in spore surface properties. The monosaccharide analysis of B. cereus ATCC 14579 showed different glycosylation patterns on ExsH and BclA. Moreover, two specific glycosyl residues, namely, 2-O-methyl-rhamnose (2-Me-Rha) and 2,4-O-methyl-rhamnose (2,4-Me-Rha), were attached to BclA, in addition to the glycosyl residues already reported in B. anthracis.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
Inserm
CNRS

Collections :

• Physiologie Cellulaire (PHYCELL) - U1003
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2019-05-16T16:44:40Z
2021-03-17T07:08:27Z
2021-03-17T07:15:32Z
2021-05-17T13:59:19Z