Titre :

Early exposure to farm dust in an allergic airway inflammation rabbit model: Does it affect bronchial and cough hyperresponsiveness?

Auteur(s) :

Divaret-Chauveau, Amandine [Auteur]
Laboratoire Chrono-environnement (UMR 6249) [LCE]
Développement, Adaptation et Handicap [DevAH]
Centre Hospitalier Régional Universitaire de Nancy [CHRU Nancy]
Foucaud, Laurent [Auteur]
Développement, Adaptation et Handicap [DevAH]
Demoulin, Bruno [Auteur]
Développement, Adaptation et Handicap [DevAH]
Teston, Cédric [Auteur]
Développement, Adaptation et Handicap [DevAH]
Loison, Pauline [Auteur]
Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) [INRS ( Vandoeuvre lès Nancy)]
Le Cann, Pierre [Auteur]
Laboratoire d'étude et de recherche en environnement et santé [LERES]
Institut de recherche en santé, environnement et travail [Irset]
Département des sciences en santé environnementale [DEESSE]
École des Hautes Études en Santé Publique [EHESP] [EHESP]
Schweitzer, Cyril [Auteur]
Centre Hospitalier Régional Universitaire de Nancy [CHRU Nancy]
Développement, Adaptation et Handicap [DevAH]
de Carvalho Bittencourt, Marcelo [Auteur]
Ingénierie Moléculaire et Physiopathologie Articulaire [IMoPA]
Centre Hospitalier Régional Universitaire de Nancy [CHRU Nancy]
Mauny, Frédéric [Auteur]
Centre d'Investigation Clinique de Besançon [Inserm CIC 1431]
Laboratoire Chrono-environnement (UMR 6249) [LCE]
Demoulin-Alexikova, Silvia [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Développement, Adaptation et Handicap [DevAH]

Titre de la revue :

PLoS One

Pagination :

e0279498

Éditeur :

Public Library of Science

Date de publication :

2023-01-27

ISSN :

1932-6203

Mot(s)-clé(s) :

Maladies respiratoires allergiques

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Santé publique et épidémiologie

Résumé en anglais : [en]

Introduction <br>Over the past 50 years, the prevalence of allergic respiratory diseases has been increasing. The Hygiene hypothesis explains this progression by the decrease in the bio-diversity of early microbial exposure. ...
Lire la suite >Introduction <br>Over the past 50 years, the prevalence of allergic respiratory diseases has been increasing. The Hygiene hypothesis explains this progression by the decrease in the bio-diversity of early microbial exposure. This study aims to evaluate the effect of early-life farm exposure on airway hyperresponsiveness and cough hypersensitivity in an allergic airway inflammation rabbit model. <br>Method <br> A specific environment was applied to pregnant rabbits and their offspring until six weeks after birth. Rabbits were housed in a pathogen-free zone for the control group and a calf barn for the farm group. At the end of the specific environmental exposure, both groups were then housed in a conventional zone and then sensitized to ovalbumin. Ten days after sensitization, the rabbit pups received ovalbumin aerosols to provoke airway inflammation. Sensitization to ovalbumin was assessed by specific IgE assay. Cough sensitivity was assessed by mechanical stimulation of the trachea, and bronchial reactivity was assessed by methacholine challenge. The farm environment was characterized by endotoxin measurement. Results A total of 38 rabbit pups were included (18 in the farm group). Endotoxin levels in the farm environment varied from 30 to 1854 EU.m -3 . There was no significant difference in specific IgE values to ovalbumin (p = 0.826) between the two groups. The mechanical threshold to elicit a cough did not differ between the two groups (p = 0.492). There was no difference in the number of cough (p = 0.270) or the intensity of ventilatory responses (p = 0.735). After adjusting for age and weight, there was no difference in respiratory resistance before and after methacholine challenge. <br>Conclusion <br> Early exposure to the calf barn did not affect cough sensitivity or bronchial reactivity in ovalbumin-sensitized rabbits. These results suggest that not all farm environments protect against asthma and atopy. Continuous exposure to several sources of microbial diversity is probably needed.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL