Solid-state NMR molecular snapshots of ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Title :
Solid-state NMR molecular snapshots of Aspergillus fumigatus cell wall architecture during a conidial morphotype transition
Author(s) :
Lamon, Gaëlle [Auteur]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Institut Européen de Chimie et Biologie [IECB]
Lends, Alons [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Valsecchi, Isabel [Auteur]
Plateforme Technologique de RMN Biologique et HDX-MS - Biological NMR and HDX-MS Technological Platform
Wong, Sarah Sze Wah [Auteur]
Mycologie moléculaire - Molecular Mycology
Duprès, Vincent [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Tolchard, James [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Schmitt, Christine [Auteur]
Plate-forme de bioimagerie ultrastructurale - Ultrastructural BioImaging Core Facility
Mallet, Adeline [Auteur]
Plate-forme de bioimagerie ultrastructurale - Ultrastructural BioImaging Core Facility
Grélard, Axelle [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Morvan, Estelle [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Dufourc, Erick [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Habenstein, Birgit [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Guijarro, J. Iñaki [Auteur]
Plateforme Technologique de RMN Biologique et HDX-MS - Biological NMR and HDX-MS Technological Platform
Aimanianda, Vishukumar [Auteur]
Mycologie moléculaire - Molecular Mycology
Loquet, Antoine [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Institut Européen de Chimie et Biologie [IECB]
Lends, Alons [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Valsecchi, Isabel [Auteur]
Plateforme Technologique de RMN Biologique et HDX-MS - Biological NMR and HDX-MS Technological Platform
Wong, Sarah Sze Wah [Auteur]
Mycologie moléculaire - Molecular Mycology
Duprès, Vincent [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Lafont, Frank [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Tolchard, James [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Schmitt, Christine [Auteur]
Plate-forme de bioimagerie ultrastructurale - Ultrastructural BioImaging Core Facility
Mallet, Adeline [Auteur]
Plate-forme de bioimagerie ultrastructurale - Ultrastructural BioImaging Core Facility
Grélard, Axelle [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Morvan, Estelle [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Dufourc, Erick [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Habenstein, Birgit [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Guijarro, J. Iñaki [Auteur]
Plateforme Technologique de RMN Biologique et HDX-MS - Biological NMR and HDX-MS Technological Platform
Aimanianda, Vishukumar [Auteur]
Mycologie moléculaire - Molecular Mycology
Loquet, Antoine [Auteur]
Institut Européen de Chimie et Biologie [IECB]
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]
Journal title :
Proceedings of the National Academy of Sciences of the United States of America
Publisher :
National Academy of Sciences
Publication date :
2023-02-07
ISSN :
0027-8424
English keyword(s) :
Aspergillus fumigatus
cell wall dynamics
conidium
germination
solid-state NMR
cell wall dynamics
conidium
germination
solid-state NMR
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, the conidial cell wall undergoes ...
Show more >While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, the conidial cell wall undergoes dynamic remodeling, which poses challenges to the host immune system and antifungal drugs. However, such cell wall reorganization during conidial germination has not been studied so far. Here, we explored the molecular rearrangement of Aspergillus fumigatus cell wall polysaccharides during different stages of germination. We took advantage of magic-angle spinning NMR to investigate the cell wall polysaccharides, without employing any destructive method for sample preparation. The breaking of dormancy was associated with a significant change in the molar ratio between the major polysaccharides β-1,3-glucan and α-1,3-glucan, while chitin remained equally abundant. The use of various polarization transfers allowed the detection of rigid and mobile polysaccharides; the appearance of mobile galactosaminogalactan was a molecular hallmark of germinating conidia. We also report for the first time highly abundant triglyceride lipids in the mobile matrix of conidial cell walls. Water to polysaccharides polarization transfers revealed an increased surface exposure of glucans during germination, while chitin remained embedded deeper in the cell wall, suggesting a molecular compensation mechanism to keep the cell wall rigidity. We complement the NMR analysis with confocal and atomic force microscopies to explore the role of melanin and RodA hydrophobin on the dormant conidial surface. Exemplified here using Aspergillus fumigatus as a model, our approach provides a powerful tool to decipher the molecular remodeling of fungal cell walls during their morphotype switching.Show less >
Show more >While establishing an invasive infection, the dormant conidia of Aspergillus fumigatus transit through swollen and germinating stages, to form hyphae. During this morphotype transition, the conidial cell wall undergoes dynamic remodeling, which poses challenges to the host immune system and antifungal drugs. However, such cell wall reorganization during conidial germination has not been studied so far. Here, we explored the molecular rearrangement of Aspergillus fumigatus cell wall polysaccharides during different stages of germination. We took advantage of magic-angle spinning NMR to investigate the cell wall polysaccharides, without employing any destructive method for sample preparation. The breaking of dormancy was associated with a significant change in the molar ratio between the major polysaccharides β-1,3-glucan and α-1,3-glucan, while chitin remained equally abundant. The use of various polarization transfers allowed the detection of rigid and mobile polysaccharides; the appearance of mobile galactosaminogalactan was a molecular hallmark of germinating conidia. We also report for the first time highly abundant triglyceride lipids in the mobile matrix of conidial cell walls. Water to polysaccharides polarization transfers revealed an increased surface exposure of glucans during germination, while chitin remained embedded deeper in the cell wall, suggesting a molecular compensation mechanism to keep the cell wall rigidity. We complement the NMR analysis with confocal and atomic force microscopies to explore the role of melanin and RodA hydrophobin on the dormant conidial surface. Exemplified here using Aspergillus fumigatus as a model, our approach provides a powerful tool to decipher the molecular remodeling of fungal cell walls during their morphotype switching.Show less >
Language :
Anglais
Popular science :
Non
ANR Project :
Source :
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