Title :

Structure-function analysis of DltE reveals D-alanylated lipoteichoic acids as direct cues supporting juvenile growth

Author(s) :

Nikolopoulos, Nikos [Auteur]
Microbiologie moléculaire et biochimie structurale - Molecular Microbiology and Structural Biochemistry [MMSB]
Matos, Renata C [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Ravaud, Stephanie [Auteur]
Microbiologie moléculaire et biochimie structurale - Molecular Microbiology and Structural Biochemistry [MMSB]
Courtin, Pascal [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Akherraz, Houssam [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]
Palussiere, Simon [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Gueguen-Chaignon, Virginie [Auteur]
EA3444
Salomon-Mallet, Marie [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Guillot, Alain [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Gifu University
Chapot-Chartier, Marie-Pierre [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Grangeasse, Christophe [Auteur]
Microbiologie moléculaire et biochimie structurale - Molecular Microbiology and Structural Biochemistry [MMSB]
Leulier, François [Auteur]
Institut de Génomique Fonctionnelle de Lyon [IGFL]

Journal title :

eLife

Volume number :

12

Publisher :

eLife Sciences Publication

Publication date :

2023-04-12

ISSN :

2050-084X

English keyword(s) :

Animals
Drosophila
Drosophila melanogaster
Teichoic Acids
Cues
Lipopolysaccharides
Biological Phenomena
D. melanogaster
Lactiplantibacillus plantarum
cell wall
infectious disease
lipoteichoic acids
microbiology
symbiosis

HAL domain(s) :

Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique

English abstract : [en]

Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial ...
Show more >Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the operon involved in 's juvenile growth promotion by . Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that lipoteichoic acids (LTAs) but not wall teichoic acids are D-alanylated in cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of and as a symbiosis model, we establish that D-alanylated LTAs (D-Ala-LTAs) are direct cues supporting intestinal peptidase expression and juvenile growth in . Our results pave the way to probing the contribution of D-Ala-LTAs to host physiology in other symbiotic models.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

ANR Project :

Symbiose bénéfique hôte-microbiote durant une sous nutrition chronique: Enveloppe bactérienne et promotion de la croissance linéaire

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Chemical Glycobiology

Submission date :

2023-07-19T08:39:29Z
2023-08-31T17:40:45Z