Autophagy Protects against Eosinophil ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
Permalink :
Title :
Autophagy Protects against Eosinophil Cytolysis and Release of DNA.
Author(s) :
Esnault, Stéphane [Auteur]
University of Wisconsin-Madison
University of Wisconsin School of Medicine and Public Health
Fichtinger, Paul S [Auteur]
Barretto, Karina T [Auteur]
Fogerty, Frances J [Auteur]
Bernau, Ksenija [Auteur]
Mosher, Deane F [Auteur]
Mathur, Sameer K [Auteur]
Sandbo, Nathan [Auteur]
Jarjour, Nizar N [Auteur]
University of Wisconsin-Madison
University of Wisconsin-Madison
University of Wisconsin School of Medicine and Public Health
Fichtinger, Paul S [Auteur]
Barretto, Karina T [Auteur]
Fogerty, Frances J [Auteur]
Bernau, Ksenija [Auteur]
Mosher, Deane F [Auteur]
Mathur, Sameer K [Auteur]
Sandbo, Nathan [Auteur]
Jarjour, Nizar N [Auteur]
University of Wisconsin-Madison
Journal title :
Cells
Abbreviated title :
Cells
Volume number :
11
Pages :
1821
Publisher :
MDPI
Publication date :
2022-06-02
ISSN :
2073-4409
English keyword(s) :
Autophagy
DNA
Eosinophils
Immunoglobulin G
Interleukin-5
DNA traps
IL3
IL5
IgG
MAP1LC3B
SQSTM1
autophagy
cytolysis
eosinophils
DNA
Eosinophils
Immunoglobulin G
Interleukin-5
DNA traps
IL3
IL5
IgG
MAP1LC3B
SQSTM1
autophagy
cytolysis
eosinophils
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
The presence of eosinophils in the airway is associated with asthma severity and risk of exacerbations. Eosinophils deposit their damaging products in airway tissue, likely by degranulation and cytolysis. We previously ...
Show more >The presence of eosinophils in the airway is associated with asthma severity and risk of exacerbations. Eosinophils deposit their damaging products in airway tissue, likely by degranulation and cytolysis. We previously showed that priming blood eosinophils with IL3 strongly increased their cytolysis on aggregated IgG. Conversely, IL5 priming did not result in significant eosinophil cytolysis in the same condition. Therefore, to identify critical events protecting eosinophils from cell cytolysis, we examined the differential intracellular events between IL5- and IL3-primed eosinophils interacting with IgG. We showed that both IL3 and IL5 priming increased the eosinophil adhesion to IgG, phosphorylation of p38, and production of reactive oxygen species (ROS), and decreased the phosphorylation of cofilin. However, autophagic flux as measured by the quantification of SQSTM1-p62 and lipidated-MAP1L3CB over time on IgG, with or without bafilomycin-A1, was higher in IL5-primed compared to IL3-primed eosinophils. In addition, treatment with bafilomycin-A1, an inhibitor of granule acidification and autophagolysosome formation, enhanced eosinophil cytolysis and DNA trap formation in IL5-primed eosinophils. Therefore, this study suggests that increased autophagy in eosinophils protects from cytolysis and the release of DNA, and thus limits the discharge of damaging intracellular eosinophilic contents.Show less >
Show more >The presence of eosinophils in the airway is associated with asthma severity and risk of exacerbations. Eosinophils deposit their damaging products in airway tissue, likely by degranulation and cytolysis. We previously showed that priming blood eosinophils with IL3 strongly increased their cytolysis on aggregated IgG. Conversely, IL5 priming did not result in significant eosinophil cytolysis in the same condition. Therefore, to identify critical events protecting eosinophils from cell cytolysis, we examined the differential intracellular events between IL5- and IL3-primed eosinophils interacting with IgG. We showed that both IL3 and IL5 priming increased the eosinophil adhesion to IgG, phosphorylation of p38, and production of reactive oxygen species (ROS), and decreased the phosphorylation of cofilin. However, autophagic flux as measured by the quantification of SQSTM1-p62 and lipidated-MAP1L3CB over time on IgG, with or without bafilomycin-A1, was higher in IL5-primed compared to IL3-primed eosinophils. In addition, treatment with bafilomycin-A1, an inhibitor of granule acidification and autophagolysosome formation, enhanced eosinophil cytolysis and DNA trap formation in IL5-primed eosinophils. Therefore, this study suggests that increased autophagy in eosinophils protects from cytolysis and the release of DNA, and thus limits the discharge of damaging intracellular eosinophilic contents.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Submission date :
2023-10-18T11:36:55Z
2023-10-25T09:18:55Z
2023-10-25T09:18:55Z
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