Titre :

Prenatal exposure to triclosan assessed in multiple urine samples and placental DNA methylation

Auteur(s) :

Jedynak, P. [Auteur]
Université Paris-Saclay
Broséus, L. [Auteur]
Tost, J. [Auteur]
Busato, F. [Auteur]
Gabet, Stephan [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Thomsen, C. [Auteur]
Sakhi, A. K. [Auteur]
Pin, I. [Auteur]
Slama, R. [Auteur]
Lepeule, J. [Auteur]
Philippat, C. [Auteur]

Titre de la revue :

Environmental Pollution

Nom court de la revue :

Environ Pollut

Numéro :

135

Pagination :

122197

Date de publication :

2023-08-08

ISSN :

1873-6424

Mot(s)-clé(s) en anglais :

Epigenetics
Imprinted genes
Placenta
DNA methylation
Pooled urine samples
Triclosan
Infinium Methylation EPIC

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

A previous study reported positive associations of maternal urinary concentrations of triclosan, a synthetic phenol with widespread exposure in the general population, with placental DNA methylation of male fetuses. Given ...
Lire la suite >A previous study reported positive associations of maternal urinary concentrations of triclosan, a synthetic phenol with widespread exposure in the general population, with placental DNA methylation of male fetuses. Given the high number of comparisons performed in -omic research, further studies were needed to validate and extend on these findings. Using a cohort of male and female fetuses with repeated maternal urine samples to assess exposure, we studied the associations between triclosan and placental DNA methylation. We assessed triclosan concentrations in two pools of 21 urine samples collected among 395 women from the SEPAGES cohort. We used Infinium Methylation EPIC arrays to measure DNA methylation in placental biopsies collected at delivery. We performed a candidate study restricted to a set of candidate CpGs (n = 500) identified in a previous work as well as an exploratory epigenome-wide association study to investigate the associations between triclosan and differentially methylated probes and regions. Analyses were conducted on the whole population and stratified by child's sex. Mediation analysis was performed to test whether heterogeneity of placental tissue may mediate the observed associations. In the candidate approach, we confirmed 18 triclosan-associated genes when both sexes were considered. After stratification for child's sex, triclosan was associated with 72 genes in females and three in males. Most of the associations were positive and several CpGs mapped to imprinted genes: FBRSL1, KCNQ1, RHOBTB3, and SMOC1. A mediation effect by placental tissue heterogeneity was identified for most of the observed associations. In the exploratory analysis, we identified a few isolated associations in the sex-stratified analysis. In line with a previous study on male placentas, our approach revealed several positive associations between triclosan exposure and placental DNA methylation. Several identified loci mapped to imprinted genes.Lire moins >

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CHU Lille
Institut Pasteur de Lille

Collections :

• IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483

Date de dépôt :

2023-10-20T05:46:30Z
2024-02-23T11:45:41Z
2024-02-23T11:48:17Z