Proteomic Characterization of Drosophila melanogaster Proboscis

Aruçi, E.; Saliou, Jean-Michel; Ferveur, J. F.; Briand, L.

Document type :

Article dans une revue scientifique: Article original

DOI :

10.3390/biology11111687

PMID :

36421401

Permalink :

http://hdl.handle.net/20.500.12210/95729

Title :

Proteomic Characterization of Drosophila melanogaster Proboscis

Author(s) :

Aruçi, E. [Auteur]
Saliou, Jean-Michel [Auteur]

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41
Ferveur, J. F. [Auteur]
Briand, L. [Auteur]

Journal title :

Biology (Basel)

Abbreviated title :

Biology (Basel)

Volume number :

Pages :

p. 1687

Publication date :

2022

ISSN :

2079-7737

English keyword(s) :

chemosensory system
taste
odorant-binding proteins
mass spectrometry
proteomics

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Drosophila melanogaster flies use their proboscis to taste and distinguish edible compounds from toxic compounds. With their proboscis, flies can detect sex pheromones at a close distance or by contact. Most of the known ...
Show more >Drosophila melanogaster flies use their proboscis to taste and distinguish edible compounds from toxic compounds. With their proboscis, flies can detect sex pheromones at a close distance or by contact. Most of the known proteins associated with probosci’s detection belong to gustatory receptor families. To extend our knowledge of the proboscis-taste proteins involved in chemo-detection, we used a proteomic approach to identify soluble proteins from Drosophila females and males. This investigation, performed with hundreds of dissected proboscises, was initiated by the chromatographic separation of tryptic peptides, followed by tandem mass spectrometry, allowing for femtomole detection sensitivity. We found 586 proteins, including enzymes, that are involved in intermediary metabolism and proteins dedicated to various functions, such as nucleic acid metabolism, ion transport, immunity, digestion, and organ development. Among 60 proteins potentially involved in chemosensory detection, we identified two odorant-binding proteins (OBPs), i.e., OBP56d (which showed much higher expression in females than in males) and OBP19d. Because OBP56d was also reported to be more highly expressed in the antennae of females, this protein can be involved in the detection of both volatile and contact male pheromone(s). Our proteomic study paves the way to better understand the complex role of Drosophila proboscis in the chemical detection of food and pheromonal compounds.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

Plateformes Lilloises en Biologie et Santé (PLBS) - UAR 2014 - US 41

Submission date :

2023-12-21T06:45:23Z
2024-02-23T11:42:24Z

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Except where otherwise noted, this item's license is described as Attribution 3.0 United States

Version	Link	Modification date
2	20.500.12210/95729*	2024-02-23T11:39:03Z
1	20.500.12210/95729.1	2023-12-21T06:45:23Z

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Proteomic Characterization of Drosophila ...

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