Title :

Transcriptomic Analysis of Breast Cancer Stem Cells and Development of a pALDH1A1:mNeptune Reporter System for Live Tracking

Author(s) :

Bidan, Nadège [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bailleul-Dubois, Justine [Auteur]
Institut pour la recherche sur le cancer de Lille [Lille] [IRCL]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Duval, Jérémy [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Winter, Marie [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Denoulet, Marie [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Hannebicque, Karine [Auteur]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Institut National de la Santé et de la Recherche Médicale [INSERM]
El-Sayed, Ihsan [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Ginestier, Christophe [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Forissier, Violaine [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Charafe-Jauffret, Emmanuelle [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Macario, Manon [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Matsunaga, Yukiko [Auteur]
Université de Lille
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Center for International Research on Integrative Biomedical Systems [University of Tokyo] [CIBiS]
Meignan, Samuel [Auteur]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Institut pour la recherche sur le cancer de Lille [Lille] [IRCL]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Anquez, François [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Julien, Sylvain [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bonnefond, Amélie [Auteur]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Derhourhi, Mehdi [Auteur]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Le Bourhis, Xuefen [Auteur]
Université Lille Nord de France (COMUE)
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Lagadec, Chann [Auteur correspondant]
Institut pour la recherche sur le cancer de Lille [Lille] [IRCL]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]

Journal title :

Proteomics

Publisher :

Wiley-VCH Verlag

Publication date :

2019-11

ISSN :

1615-9853

English keyword(s) :

cancer stem cell
tracking

HAL domain(s) :

Sciences du Vivant [q-bio]/Cancer

English abstract : [en]

Abstract Many solid cancers are hierarchically organized with a small number of cancer stem cells (CSCs) able to regrow a tumor, while their progeny lacks this feature. Breast CSC is known to contribute to therapy resistance. ...
Show more >Abstract Many solid cancers are hierarchically organized with a small number of cancer stem cells (CSCs) able to regrow a tumor, while their progeny lacks this feature. Breast CSC is known to contribute to therapy resistance. The study of those cells is usually based on their cell‐surface markers like CD44 high /CD24 low/neg or their aldehyde dehydrogenase (ALDH) activity. However, these markers cannot be used to track the dynamics of CSC. Here, a transcriptomic analysis is performed to identify segregating gene expression in CSCs and non‐CSCs, sorted by Aldefluor assay. It is observed that among ALDH‐associated genes, only ALDH1A1 isoform is increased in CSCs. A CSC reporter system is then developed by using a far red‐fluorescent protein (mNeptune) under the control of ALDH1A1 promoter. mNeptune‐positive cells exhibit higher sphere‐forming capacity, tumor formation, and increased resistance to anticancer therapies. These results indicate that the reporter identifies cells with stemness characteristics. Moreover, live tracking of cells in a microfluidic system reveals a higher extravasation potential of CSCs. Live tracking of non‐CSCs under irradiation treatment show, for the first time, live reprogramming of non‐CSCs into CSCs. Therefore, the reporter will allow for cell tracking to better understand the implication of CSCs in breast cancer development and recurrence.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

ANR Project :

Plate forme Lilloise de séquençage du génome humain pour une médecine personnalisée
EGID Diabetes Pole

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL