Title :

Methodological considerations for ghrelin isoforms assay in clinical evaluation in anorexia nervosa.

Author(s) :

Tezenas du Montcel, C [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
Duriez, P [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
Lebrun, N [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
Grouselle, D [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
de Grimaudet, B [Auteur]
Dardennes, R [Auteur]
Université Paris Cité [UPCité]
Epelbaum, J [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
Cuenca, M [Auteur]
Viltart, Odile [Auteur]
Laboratoire Sciences Cognitives et Sciences Affectives - UMR 9193 [SCALab]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
Gorwood, P [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]
Tolle, V [Auteur]
Institut de psychiatrie et neurosciences de Paris [IPNP - U1266 Inserm]

Journal title :

Comprehensive Psychoneuroendocrinology

Abbreviated title :

Compr Psychoneuroendocrinol

Volume number :

11

Pages :

100140

Publication date :

2022-05-20

ISSN :

2666-4976

English keyword(s) :

Anorexia nervosa
Appetite
Ghrelin
Immunoassay
Pulsatile secretion

HAL domain(s) :

Sciences cognitives

English abstract : [en]

The growing interest concerning the role of metabolic sensors in various eating disorders requires the implementation of a strict methodology to collect, store and process blood samples in clinical studies. In particular, ...
Show more >The growing interest concerning the role of metabolic sensors in various eating disorders requires the implementation of a strict methodology to collect, store and process blood samples in clinical studies. In particular, measurement of isoforms of the appetite-stimulating hormone, ghrelin, has been challenging in clinical settings. Indeed the acyl ghrelin (AG) isoform is rapidly degraded into desacyl ghrelin (DAG) by blood esterases, thus optimal conditions for the conservation of AG and accurate determination of AG/DAG ratio should be used. Here, we compared different protease inhibitors (Aprotinin, PHMB, AEBSF) during blood collection, increasing delays (0-180 min) before centrifugation, plasma supplementation with various HCl concentrations, storage durations of frozen plasma (8 and 447 days) and immunoenzyme-assay procedures (one-step sequential) in healthy subjects. Optimal conditions were obtained by collecting blood with aprotinin and supplementation of plasma with 0.1 N HCl with subsequent freezing for at least 8 days and using one-step assay. Under such conditions, different patterns of secretion of ghrelin isoforms were characterized in patients with restrictive-type anorexia nervosa (AN-R) before and after nutritional recovery. We illustrate the pulsatile variations of ghrelin isoforms according to the time around a meal and hunger rates in 3 patients with AN-R. This study offers a comprehensive comparison of various conditions using selective and specific immunoassays for both ghrelin isoforms in order to optimize assay sensitivity and consistency among procedures. These assay conditions could therefore be widely used to elucidate precisely the role of ghrelin isoforms on eating behavior in physiological and pathological situations.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
CHU Lille

Collections :

• Sciences Cognitives et Sciences Affectives (SCALab) - UMR 9193

Research team(s) :

Équipe Dynamique Émotionnelle et Pathologies (DEEP)

Submission date :

2024-01-10T15:01:01Z
2024-01-10T18:17:05Z
2024-01-10T18:17:58Z