Title :

The Sterilization of Human Milk by Holder Pasteurization or by High Hydrostatic Pressure Processing Leads to Differential Intestinal Effects in Mice.

Author(s) :

Carneiro, Lionel [Auteur]
Centre Hospitalier Universitaire de Toulouse [CHU Toulouse]
NeuroMicrobiota
Marousez, Lucie [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]
Van Hul, Matthias [Auteur]
NeuroMicrobiota
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Walloon Excellence in Life sciences and BIOtechnology [Liège] [WELBIO]
De Lamballerie, Marie [Auteur]
Laboratoire de génie des procédés - environnement - agroalimentaire [GEPEA]
Ley, Delphine [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Cani, Patrice D. [Auteur]
NeuroMicrobiota
Université Catholique de Louvain = Catholic University of Louvain [UCL]
Walloon Excellence in Life sciences and BIOtechnology [Liège] [WELBIO]
Knauf, Claude [Auteur]
Centre Hospitalier Universitaire de Toulouse [CHU Toulouse]
NeuroMicrobiota
Lesage, Jean [Auteur]
Institute for Translational Research in Inflammation - U 1286 [INFINITE]

Journal title :

Nutrients

Abbreviated title :

Nutrients

Volume number :

15

Publication date :

2023-10-03

ISSN :

2072-6643

English keyword(s) :

human milk
high hydrostatic pressure processing
holder pasteurization
mice
gut barrier
microbiota

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Background: Human milk banks (HMBs) provide sterilized donor milk (DM) for the feeding of preterm infants. Most HMBs use the standard method of Holder pasteurization (HoP) performed by heating DM at 62.5 °C for 30 min. ...
Show more >Background: Human milk banks (HMBs) provide sterilized donor milk (DM) for the feeding of preterm infants. Most HMBs use the standard method of Holder pasteurization (HoP) performed by heating DM at 62.5 °C for 30 min. High hydrostatic pressure (HHP) processing has been proposed as an alternative to HoP. This study aims to evaluate intestinal barrier integrity and microbiota composition in adult mice subjected to a chronic oral administration of HoP- or HHP-DM. Methods: Mice were treated by daily gavages with HoP- or HHP-DM over seven days. Intestinal barrier integrity was assessed through in vivo 4 kDa FITC–dextran permeability assay and mRNA expression of several tight junctions and mucins in ileum and colon. Cecal short chain fatty acids (SCFAs) and microbiota were analyzed. Results: HHP-DM mice displayed decreased intestinal permeability to FITC–dextran and increased ileal mRNA expression levels of two tight junctions (Ocln and Cdh1) and Muc2. In the colon, mRNA expression levels of two tight junctions (Cdh1 and Tjp1) and of two mucins (Muc2 and Muc4) were decreased in HHP-DM mice. Cecal SCFAs and microbiota were not different between groups. Conclusions: HHP processing of DM reinforces intestinal barrier integrity in vivo without affecting gut microbiota and SCFAs production. This study reinforces previous findings showing that DM sterilization through HHP might be beneficial for the intestinal maturation of preterm infants compared with the use of HoP for the treatment of DM.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2024-01-11T22:12:20Z
2024-03-26T12:43:23Z