In Vivo Assessment of Antioxidant Potential ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
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Title :
In Vivo Assessment of Antioxidant Potential of Human Milk Treated by Holder Pasteurization or High Hydrostatic Pressure Processing: A Preliminary Study on Intestinal and Hepatic Markers in Adult Mice.
Author(s) :
Wemelle, Eve [Auteur]
NeuroMicrobiota
1002454|||Institut de Recherche en Santé Digestive [IRSD ] (VALID)
Marousez, Lucie [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Lesage, Jean [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
De Lamballerie, Marie [Auteur]
Laboratoire de génie des procédés - environnement - agroalimentaire [GEPEA]
École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique [ONIRIS]
Knauf, Claude [Auteur]
NeuroMicrobiota
Institut de Recherche en Santé Digestive [IRSD]
Carneiro, Lionel [Auteur]
NeuroMicrobiota
Institut de Recherche en Santé Digestive [IRSD]
NeuroMicrobiota
1002454|||Institut de Recherche en Santé Digestive [IRSD ] (VALID)
Marousez, Lucie [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Lesage, Jean [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
De Lamballerie, Marie [Auteur]
Laboratoire de génie des procédés - environnement - agroalimentaire [GEPEA]
École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique [ONIRIS]
Knauf, Claude [Auteur]
NeuroMicrobiota
Institut de Recherche en Santé Digestive [IRSD]
Carneiro, Lionel [Auteur]
NeuroMicrobiota
Institut de Recherche en Santé Digestive [IRSD]
Journal title :
Antioxidants
Abbreviated title :
Antioxidants (Basel)
Volume number :
11
Pages :
1091
Publication date :
2022-07-01
ISSN :
2076-3921
English keyword(s) :
human milk
high hydrostatic pressure
holder pasteurization
H2O2
antioxidants
oxidative stress
inflammation
gut
liver
high hydrostatic pressure
holder pasteurization
H2O2
antioxidants
oxidative stress
inflammation
gut
liver
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Preterm infants are highly susceptible to oxidative stress due to an imbalance between endogenous oxidant and antioxidant systems. In addition, these newborns are frequently fed with donor milk (DM) treated by Holder ...
Show more >Preterm infants are highly susceptible to oxidative stress due to an imbalance between endogenous oxidant and antioxidant systems. In addition, these newborns are frequently fed with donor milk (DM) treated by Holder pasteurization (HoP) at 62.5 °C for 30 min, which is known to alter numerous heat-sensitive factors, including some antioxidants. High hydrostatic pressure (HHP) processing was recently proposed as an innovative method for the treatment of DM. The present study aimed to measure the redox balance of HoP- and HHP-DM and to study, in vivo, the effects of HoP- and HHP-DM on the gut and liver. H2O2, vitamin A and vitamin E (α- and γ-tocopherols) concentrations, as well as the total antioxidant capacity (TAC), were measured in raw-, HoP- and HHP-DM. The gene expression level of antioxidant systems and inflammatory response were quantified in the ileum and liver of adult mice after 7 days of oral administration of HoP- or HHP-DM. HoP reduced the γ-tocopherol level, whereas HHP treatment preserved all vitamins close to the raw milk level. The milk H2O2 content was reduced by HHP but not by HoP. The total antioxidant capacity of DM was reduced after HHP processing measured by PAOT-Liquid® technology but was unaffected after measurement by ORAC assay. In mice, HHP-DM administration induced a stimulation of antioxidant defenses and reduced some inflammatory markers in both the ileum and liver compared to HoP-DM treatment. Our preliminary study suggests that the HHP processing of DM may better protect preterm infants from gut and liver pathologies compared to HoP, which is currently used in most human milk banks.Show less >
Show more >Preterm infants are highly susceptible to oxidative stress due to an imbalance between endogenous oxidant and antioxidant systems. In addition, these newborns are frequently fed with donor milk (DM) treated by Holder pasteurization (HoP) at 62.5 °C for 30 min, which is known to alter numerous heat-sensitive factors, including some antioxidants. High hydrostatic pressure (HHP) processing was recently proposed as an innovative method for the treatment of DM. The present study aimed to measure the redox balance of HoP- and HHP-DM and to study, in vivo, the effects of HoP- and HHP-DM on the gut and liver. H2O2, vitamin A and vitamin E (α- and γ-tocopherols) concentrations, as well as the total antioxidant capacity (TAC), were measured in raw-, HoP- and HHP-DM. The gene expression level of antioxidant systems and inflammatory response were quantified in the ileum and liver of adult mice after 7 days of oral administration of HoP- or HHP-DM. HoP reduced the γ-tocopherol level, whereas HHP treatment preserved all vitamins close to the raw milk level. The milk H2O2 content was reduced by HHP but not by HoP. The total antioxidant capacity of DM was reduced after HHP processing measured by PAOT-Liquid® technology but was unaffected after measurement by ORAC assay. In mice, HHP-DM administration induced a stimulation of antioxidant defenses and reduced some inflammatory markers in both the ileum and liver compared to HoP-DM treatment. Our preliminary study suggests that the HHP processing of DM may better protect preterm infants from gut and liver pathologies compared to HoP, which is currently used in most human milk banks.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Submission date :
2024-01-12T01:12:10Z
2024-03-15T09:55:18Z
2024-03-15T09:55:18Z
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