Title :

Chemical stimulation of visceral afferents activates medullary neurones projecting to the central amygdala and periaqueductal grey.

Author(s) :

Viltart, Odile [Auteur]
Institut Pasteur de Lille
Sartor, Daniela M [Auteur]
Verberne, Anthony J M [Auteur]

Journal title :

Brain Research Bulletin

Abbreviated title :

Brain Res Bull

Volume number :

71

Pages :

9-51

Publication date :

2006-12-11

ISSN :

0361-9230

English keyword(s) :

Amygdala
Animals
Blood Pressure
Cell Count
Cholecystokinin
Cholera Toxin
Gastrointestinal Tract
Immunohistochemistry
Male
Medulla Oblongata
Neurons
Periaqueductal Gray
Proto-Oncogene Proteins c-fos
Rats
Rats, Sprague-Dawley
Reticular Formation
Solitary Nucleus
Vagus Nerve
Visceral Afferents

HAL domain(s) :

Sciences cognitives

English abstract : [en]

Cholecystokinin (CCK) stimulates gastrointestinal vagal afferent neurones that signal visceral sensations. We wished to determine whether neurones of the nucleus of the solitary tract (NTS) or ventrolateral medulla (VLM) ...
Show more >Cholecystokinin (CCK) stimulates gastrointestinal vagal afferent neurones that signal visceral sensations. We wished to determine whether neurones of the nucleus of the solitary tract (NTS) or ventrolateral medulla (VLM) convey visceral afferent information to the central nucleus of the amygdala (CeA) or periaqueductal grey region (PAG), structures that play a key role in adaptive autonomic responses triggered by stress or fear. Male Sprague-Dawley rats received a unilateral microinjection of the tracer cholera toxin subunit B (CTB, 1%) into the CeA or PAG followed, 7 days later, by an injection of CCK (100 microg/kg, i.p.) or saline. Brains were processed for detection of Fos protein (Fos-IR) and CTB. CCK induced increased expression of Fos-IR in the NTS and the VLM, relative to control. When CTB was injected into the CeA, CTB-immunoreactive (CTB-IR) neurones were more numerous in the rostral NTS ipsilateral to the injection site, whereas they were homogeneously distributed throughout the VLM. Double-labelled neurones (Fos-IR+CTB-IR) were most numerous in the ipsilateral NTS and caudal VLM. The NTS contained the higher percentage of CTB-IR neurones activated by CCK. When CTB was injected into the PAG, CTB-IR neurones were more numerous in the ipsilateral NTS whereas they were distributed relatively evenly bilaterally in the rostral VLM. Double-labelled neurones were not differentially distributed along the rostrocaudal axis of the NTS but were more numerous in this structure when compared with the VLM. NTS and VLM neurones may convey visceral afferent information to the CeA and the PAG.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
CHU Lille

Collections :

• Autres travaux scientifiques

Submission date :

2024-01-12T14:55:41Z
2024-02-23T13:18:58Z