Title :

Modulation of IGF2 Expression in the Murine Thymus and Thymic Epithelial Cells Following Coxsackievirus-B4 Infection.

Author(s) :

Michaux, Hélène [Auteur]
GIGA [Université Liège]
Halouani, Aymen [Auteur]
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] [LR99ES27]
Trussart, Charlotte [Auteur]
GIGA [Université Liège]
Renard, Chantal [Auteur]
GIGA-Neurosciences [Université Liège]
Jaïdane, Hela [Auteur]
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] [LR99ES27]
Martens, Henri [Auteur]
GIGA [Université Liège]
Geenen, Vincent [Auteur]
GIGA [Université Liège]
Hober, Didier [Auteur]
Laboratoire de virologie - ULR 3610

Journal title :

Microorganisms

Abbreviated title :

Microorganisms

Volume number :

9

Pages :

402

Publication date :

2021-03-07

ISSN :

2076-2607

English keyword(s) :

thymus
thymic epithelial cell
type 1 diabetes
coxsackievirus B4
insulin-like growth factor 2
transcription
picornavirus

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Coxsackievirus B4 (CV-B4) can infect human and murine thymic epithelial cells (TECs). In a murine TEC cell line, CV-B4 can downregulate the transcription of the insulin-like growth factor 2 (Igf2) gene coding for the ...
Show more >Coxsackievirus B4 (CV-B4) can infect human and murine thymic epithelial cells (TECs). In a murine TEC cell line, CV-B4 can downregulate the transcription of the insulin-like growth factor 2 (Igf2) gene coding for the self-peptide of the insulin family. In this study, we show that CV-B4 infections of a murine TEC cell line decreased Igf2 P3 promoter activity by targeting a region near the transcription start site; however, the stability of Igf2 transcripts remained unchanged, indicating a regulation of Igf2 transcription. Furthermore, CV-B4 infections decreased STAT3 phosphorylation in vitro. We also showed that mice infected with CV-B4 had an altered expression of Igf2 isoforms as detected in TECs, followed by a decrease in the pro-IGF2 precursor in the thymus. Our study sheds new light on the intrathymic regulation of Igf2 transcription during CV-B4 infections and supports the hypothesis that a viral infection can disrupt central self-tolerance to insulin by decreasing Igf2 transcription in the thymic epithelium.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

• Laboratoire de virologie - ULR 3610

Submission date :

2024-01-17T22:16:45Z
2024-02-08T14:00:03Z