Title :

Infection of Mice With Coxsackievirus B4.

Author(s) :

Halouani, Aymen [Auteur]
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] [LR99ES27]
Jmii, Habib [Auteur]
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] [LR99ES27]
Bodart, Gwennaëlle [Auteur]
GIGA [Université Liège]
Michaux, Hélène [Auteur]
GIGA [Université Liège]
Renard, Chantal [Auteur]
GIGA-Neurosciences [Université Liège]
Martens, Henri [Auteur]
GIGA [Université Liège]
Aouni, Mahjoub [Auteur]
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] [LR99ES27]
Hober, Didier [Auteur]
Laboratoire de virologie - ULR 3610
Geenen, Vincent [Auteur]
GIGA [Université Liège]
Jaïdane, Hela [Auteur]
Laboratoire des Maladies Transmissibles et Substances Biologiquement Actives [Monastir] [LR99ES27]

Journal title :

Frontiers in Immunology

Abbreviated title :

Front Immunol

Volume number :

11

Pages :

481

Publication date :

2020-04-18

ISSN :

1664-3224

English keyword(s) :

thymus
T cell
thymic export
T cell receptor (TCR) rearrangement excision circles
autoimmune diseases
Ptk7 gene
coxsackievirus B
vertical transmission

HAL domain(s) :

Sciences du Vivant [q-bio]/Médecine humaine et pathologie

English abstract : [en]

The thymus is the main organ of the lymphatic system, in which T cells undergo a rigorous selection to ensure that their receptors (TCRs) will be functional and will not react against the self. Genes encoding for TCR chains ...
Show more >The thymus is the main organ of the lymphatic system, in which T cells undergo a rigorous selection to ensure that their receptors (TCRs) will be functional and will not react against the self. Genes encoding for TCR chains are fragmented and must be rearranged by a process of somatic recombination generating TCR rearrangement excision circles (TRECs). We recently documented coxsackievirus B4 (CV-B4) infection of Swiss albino mouse thymus in the course of in utero transmission. In the current study, we intended to evaluate thymic output in this experimental model. For this purpose, pregnant Swiss albino mice were inoculated with CV-B4 at day 10 or 17 of gestation, and thymus and spleen were sampled from offspring at different time points and then subjected to quantification of TREC molecules and Ptk7 gene expression. Results showed a pronounced effect of in utero CV-B4 infection on the thymus with an increase in the cellularity and, consequently, the weight of the organ. sj and DβTREC analysis, by real-time PCR, revealed a significant decrease following CV-B4 infection compared to controls, a decrease which gets worse as time goes by, both in the thymus and in the periphery. Those observations reflect a disturbance in the export of T cells to the periphery and their accumulation within the thymus. The evaluation of Ptk7 transcripts in the thymus, for its part, showed a decrease in expression, especially following an infection at day 10 of gestation, which supports the hypothesis of T cell accumulation in a mature stage in the thymus. The various effects observed correlate either negatively or positively with the viral load in the thymus and spleen. Disruption in thymic export may indeed interfere with T cell maturation. We speculate that this may lead to a premature release of T cells and the possibility of circulating autoreactive or proliferation-impaired T cell clones.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CHU Lille

Collections :

• Laboratoire de virologie - ULR 3610

Submission date :

2024-01-17T22:23:35Z
2024-02-13T12:09:34Z