Targeted optical imaging of the glucagon-like ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Targeted optical imaging of the glucagon-like peptide 1 receptor using exendin-4-IRDye800CW.
Author(s) :
Boss, Marti [Auteur]
Bos, Desiree [Auteur]
Frielink, Cathelijne [Auteur]
Sandker, Gerwin [Auteur]
Ekim, Selen [Auteur]
Marciniak, Camille [Auteur]
Recherche translationnelle sur le diabète - U 1190 [RTD]
Pattou, Francois [Auteur]
Recherche translationnelle sur le diabète (RTD) - U1190
Van Dam, Go M. [Auteur]
Van Lith, Sanne [Auteur]
Brom, Maarten [Auteur]
Gotthardt, Martin [Auteur]
Buitinga, Mijke [Auteur]
Bos, Desiree [Auteur]
Frielink, Cathelijne [Auteur]
Sandker, Gerwin [Auteur]
Ekim, Selen [Auteur]
Marciniak, Camille [Auteur]
Recherche translationnelle sur le diabète - U 1190 [RTD]
Pattou, Francois [Auteur]

Recherche translationnelle sur le diabète (RTD) - U1190
Van Dam, Go M. [Auteur]
Van Lith, Sanne [Auteur]
Brom, Maarten [Auteur]
Gotthardt, Martin [Auteur]
Buitinga, Mijke [Auteur]
Journal title :
Journal of Nuclear Medicine
Abbreviated title :
J. Nucl. Med.
Volume number :
61
Pages :
1066-1071
Publication date :
2020-07
ISSN :
1535-5667
English keyword(s) :
optical imaging
exendin
insulinoma
congenital hyperinsulinism
fluorescence
exendin
insulinoma
congenital hyperinsulinism
fluorescence
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
The treatment of choice for insulinomas and focal lesions in congenital hyperinsulinism (CHI) is surgery. However, intraoperative detection can be challenging. This challenge could be overcome with intraoperative fluorescence ...
Show more >The treatment of choice for insulinomas and focal lesions in congenital hyperinsulinism (CHI) is surgery. However, intraoperative detection can be challenging. This challenge could be overcome with intraoperative fluorescence imaging, which provides real-time lesion detection with a high spatial resolution. Here, a novel method for targeted near-infrared (NIR) fluorescence imaging of glucagonlike peptide 1 receptor (GLP-1R)–positive lesions, using the GLP-1 agonist exendin-4 labeled with IRDye 800CW, was examined in vitro and in vivo. Methods: A competitive binding assay was performed using Chinese hamster lung (CHL) cells transfected with GLP-1R. Tracer biodistribution was determined in BALB/c nude mice bearing subcutaneous CHL-GLP-1R xenografts. In vivo NIR fluorescence imaging of CHL-GLP-1R xenografts was performed. Localization of the tracer in the pancreatic islets of BALB/c nude mice was examined using fluorescence microscopy. Laparoscopic imaging was performed to detect the fluorescent signal of the tracer in the pancreas of mini pigs. Results: Exendin-4-IRDye 800CW binds GLP-1R with a half-maximal inhibitory concentration of 3.96 nM. The tracer accumulates in CHL-GLP-1R xenografts. Subcutaneous CHL-GLP-1R xenografts were visualized using in vivo NIR fluorescence imaging. The tracer accumulates specifically in the pancreatic islets of mice, and a clear fluorescent signal was detected in the pancreas of mini pigs. Conclusion: These data provide the first in vivo evidence of the feasibility of targeted fluorescence imaging of GLP-1R–positive lesions. Intraoperative lesion delineation using exendin-4-IRDye 800CW could benefit open as well as laparoscopic surgical procedures for removal of insulinomas and focal lesions in CHI.Show less >
Show more >The treatment of choice for insulinomas and focal lesions in congenital hyperinsulinism (CHI) is surgery. However, intraoperative detection can be challenging. This challenge could be overcome with intraoperative fluorescence imaging, which provides real-time lesion detection with a high spatial resolution. Here, a novel method for targeted near-infrared (NIR) fluorescence imaging of glucagonlike peptide 1 receptor (GLP-1R)–positive lesions, using the GLP-1 agonist exendin-4 labeled with IRDye 800CW, was examined in vitro and in vivo. Methods: A competitive binding assay was performed using Chinese hamster lung (CHL) cells transfected with GLP-1R. Tracer biodistribution was determined in BALB/c nude mice bearing subcutaneous CHL-GLP-1R xenografts. In vivo NIR fluorescence imaging of CHL-GLP-1R xenografts was performed. Localization of the tracer in the pancreatic islets of BALB/c nude mice was examined using fluorescence microscopy. Laparoscopic imaging was performed to detect the fluorescent signal of the tracer in the pancreas of mini pigs. Results: Exendin-4-IRDye 800CW binds GLP-1R with a half-maximal inhibitory concentration of 3.96 nM. The tracer accumulates in CHL-GLP-1R xenografts. Subcutaneous CHL-GLP-1R xenografts were visualized using in vivo NIR fluorescence imaging. The tracer accumulates specifically in the pancreatic islets of mice, and a clear fluorescent signal was detected in the pancreas of mini pigs. Conclusion: These data provide the first in vivo evidence of the feasibility of targeted fluorescence imaging of GLP-1R–positive lesions. Intraoperative lesion delineation using exendin-4-IRDye 800CW could benefit open as well as laparoscopic surgical procedures for removal of insulinomas and focal lesions in CHI.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
Inserm
CHU Lille
Inserm
CHU Lille
Collections :
Submission date :
2024-01-19T23:36:44Z
2024-10-30T13:39:17Z
2024-10-30T13:39:17Z
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