Title :

Exploring fetal response to acidosis in ewes: choosing an adequate experimental model

Author(s) :

Garabedian, Charles [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
170262|||Département d'obstétrique[Lille] (VALID)
Aubry, Estelle [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Environnement Périnatal et Santé - EA 4489
Sharma, Dyuti [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Environnement Périnatal et Santé - EA 4489
Bleu, G. [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Département d'obstétrique[Lille]
Clermont-Hama, Y. [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Département d'obstétrique[Lille]
Ghesquière, Louise [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Département d'obstétrique[Lille]
Hubert, Thomas [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Deruelle, P. [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Département d'obstétrique[Lille]
Storme, Laurent [Auteur]
Centre Hospitalier Régional Universitaire [CHU Lille] [CHRU Lille]
Environnement Périnatal et Santé - EA 4489
De Jonckheere, Julien [Auteur]
Environnement périnatal et croissance - EA 4489 [EPS]
Centre d'Investigation Clinique - Innovation Technologique de Lille - CIC 1403 - CIC 9301 [CIC Lille]
Debarge, Veronique [Auteur]
Département d'obstétrique[Lille]
Environnement Périnatal et Santé - EA 4489

Journal title :

Journal of Gynecology Obstetrics and Human Reproduction

Abbreviated title :

J Gynecol Obstet Hum Reprod

Volume number :

47

Pages :

397-403

Publisher :

Elsevier

Publication date :

2018-04-11

ISSN :

2468-7847

English keyword(s) :

Baroreflex
Autonomic nervous system
Acidosis
Lamb
Fetal heart rate
Experimental study

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Introduction Knowledge of fetal physiology during labor has been largely generated from animal models. Our team recently developed a new index to assess parasympathetic activity using different experimental protocols to ...
Show more >Introduction Knowledge of fetal physiology during labor has been largely generated from animal models. Our team recently developed a new index to assess parasympathetic activity using different experimental protocols to obtain acidosis. The objective of the present study was to discuss the different protocols and to review other models proposed in the literature. Material and methods Pregnant ewes underwent a surgical procedure at the 123 ± 2 days gestational age (term = 145 days). Three experimental protocols were used: protocol A consisted of 25%, 50% and 75% umbilical cord occlusion (UCO) for 20 min. Protocol B consisted of partial 75% UCO until reaching a pH < 7.10. Protocol C consisted of brief, repetitive complete occlusion until severe acidosis occurred. Hemodynamic and blood gas parameters were compared to those of the stability period before UCO. Results Protocol A led to a progressive response depending on the degree of occlusion (decrease in fetal heart rate, arterial hypertension and pH). Protocol B led to severe acidosis, although the duration of UCO varied per animal. Protocol C also progressively led to acidosis. We observed high inter individual variability in the acidosis response. Conclusion Pregnant ewes are a relevant model for exploring fetal response to acidosis. The frequency of UCO and partial or complete occlusion should be adapted to the expected effects. Knowledge of these protocols is important to respect ethical guidelines and to reduce the required number of animals. Moreover, it is important to consider the high individual variability of the acidosis response in the interpretation of the results.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
Inserm
CHU Lille

Collections :

• Environnement Périnatal et Santé - ULR 4489

Submission date :

2024-01-20T00:02:02Z
2024-04-25T14:48:12Z