Titre :

Antibodies as Models and Tools to Decipher Candida albicans Pathogenic Development: Review about a Unique Monoclonal Antibody Reacting with Immunomodulatory Adhesins

Auteur(s) :

Leroy, Jordan [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Service de Parasitologie-Mycologie [CHRU LIlle]
Centre d'Epidémiologie et de Recherche en santé des POPulations [CERPOP]
Lecointe, Karine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Centre d'Epidémiologie et de Recherche en santé des POPulations [CERPOP]
Coulon, Pauline [Auteur]
Service de Parasitologie-Mycologie [CHRU LIlle]
Sendid, Boualem [Auteur]
Service de Parasitologie-Mycologie [CHRU LIlle]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Centre d'Epidémiologie et de Recherche en santé des POPulations [CERPOP]
Robert, Raymond [Auteur]
Poulain, Daniel [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Centre d'Epidémiologie et de Recherche en santé des POPulations [CERPOP]

Titre de la revue :

Journal of Fungi

Nom court de la revue :

JoF

Numéro :

9

Pagination :

636

Éditeur :

MDPI

Date de publication :

2023-05-31

ISSN :

2309-608X

Mot(s)-clé(s) en anglais :

monoclonal antibody
immunity
Candida albicans
diagnosis

Discipline(s) HAL :

Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

Candidiasis, caused mainly by Candida albicans, a natural commensal of the human digestive tract and vagina, is the most common opportunistic fungal infection at the mucosal and systemic levels. Its high morbi–mortality ...
Lire la suite >Candidiasis, caused mainly by Candida albicans, a natural commensal of the human digestive tract and vagina, is the most common opportunistic fungal infection at the mucosal and systemic levels. Its high morbi–mortality rates have led to considerable research to identify the molecular mechanisms associated with the switch to pathogenic development and to diagnose this process as accurately as possible. Since the 1980s, the advent of monoclonal antibody (mAb) technology has led to significant progress in both interrelated fields. This linear review, intended to be didactic, was prompted by considering how, over several decades, a single mAb designated 5B2 contributed to the elucidation of the molecular mechanisms of pathogenesis based on β-1,2-linked oligomannoside expression in Candida species. These contributions starting from the structural identification of the minimal epitope as a di-mannoside from the β-1,2 series consisted then in the demonstration that it was shared by a large number of cell wall proteins differently anchored in the cell wall and the discovery of a cell wall glycoplipid shed by the yeast in contact of host cells, the phospholipomannan. Cytological analysis revealed an overall highly complex epitope expression at the cell surface concerning all growth phases and a patchy distribution resulting from the merging of cytoplasmic vesicles to plasmalema and further secretion through cell wall channels. On the host side, the mAb 5B2 led to identification of Galectin-3 as the human receptor dedicated to β-mannosides and signal transduction pathways leading to cytokine secretion directing host immune responses. Clinical applications concerned in vivo imaging of Candida infectious foci, direct examination of clinical samples and detection of circulating serum antigens that complement the Platelia Ag test for an increased sensitivity of diagnosis. Finally, the most interesting character of mAb 5B2 is probably its ability to reveal C. albicans pathogenic behaviour in reacting specifically with vaginal secretions from women infected versus colonized by this species as well as to display higher reactivity with strains isolated in pathogenic circumstances or even linked to an unfavourable prognosis for systemic candidiasis. Together with a detailed referenced description of these studies, the review provides a complementary reading frame by listing the wide range of technologies involving mAb 5B2 over time, evidencing a practical robustness and versatility unique so far in the Candida field. Finally, the basic and clinical perspectives opened up by these studies are briefly discussed with regard to prospects for future applications of mAb 5B2 in current research challenges.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Autre(s) projet(s) ou source(s) de financement :

INSERM

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Glycobiology in fungal Pathogenesis and Clinical Applications

Date de dépôt :

2024-01-23T15:24:25Z
2024-01-25T14:03:15Z
2024-01-25T14:04:24Z