Title :

Inflammation and er stress differentially regulate stamp2 expression and localization in adipocytes

Author(s) :

Sikkeland, Jorgen [Auteur]
Lindstad, Torstein [Auteur]
Nenseth, Hatice Zeynep [Auteur]
Dezitter, Xavier [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Qu, Su [Auteur]
Muhumed, Ridhwan M. [Auteur]
Ertunc, Meric Erikci [Auteur]
Gregor, Margaret F. [Auteur]
Saatcioglu, Fahri [Auteur]

Journal title :

Metabolism. clinical and experimental

Abbreviated title :

Metab. Clin. Exp.

Publication date :

2019-01-30

ISSN :

1532-8600

English keyword(s) :

Iron reductase
STAMP2
Adipocyte
Protein translocation
Gene expression
ER stress

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Chronic ER stress and dysfunction is a hallmark of obesity and a critical contributor to metaflammation, abnormal hormone action and altered substrate metabolism in metabolic tissues, such as liver and adipocytes. Lack of ...
Show more >Chronic ER stress and dysfunction is a hallmark of obesity and a critical contributor to metaflammation, abnormal hormone action and altered substrate metabolism in metabolic tissues, such as liver and adipocytes. Lack of STAMP2 in lean mice induces inflammation and insulin resistance on a regular diet, and it is dysregulated in the adipose tissue of obese mice and humans. We hypothesized that the regulation of STAMP2 is disrupted by ER stress. 3T3-L1 and MEF adipocytes were treated with ER stress inducers thapsigargin and tunicamycin, and inflammation inducer TNFα. The treatments effect on STAMP2 expression and enzymatic function was assessed. In addition, 3T3-L1 adipocytes and HEK cells were utilized for Stamp2 promoter activity investigation performed with luciferase and ChIP assays. ER stress significantly reduced both STAMP2 mRNA and protein expression in cultured adipocytes whereas TNFα had the opposite effect. Concomitant with loss of STAMP2 expression during ER stress, intracellular localization of STAMP2 was altered and total iron reductase activity was reduced. Stamp2 promoter analysis by reporter assays and chromatin immunoprecipitation, showed that induction of ER stress disrupts C/EBPα-mediated STAMP2 expression. These data suggest a clear link between ER stress and quantitative and functional STAMP2-deficiency.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2024-01-30T10:27:32Z