Title :

Promising antibacterial efficacy of arenicin peptides against the emerging opportunistic pathogen Mycobacterium abscessus

Author(s) :

Casanova, Magali [Auteur]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Maresca, Marc [Auteur]
Institut des Sciences Moléculaires de Marseille [ISM2]
Poncin, Isabelle [Auteur]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Point, Vanessa [Auteur]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Olleik, Hamza [Auteur]
Institut des Sciences Moléculaires de Marseille [ISM2]
Wichlacz, Celine [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Tasiemski, Aurélie [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Mabrouk, Kamel [Auteur]
Institut de Chimie Radicalaire [ICR]
Cavalier, Jean-François [Auteur correspondant]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]
Canaan, Stéphane [Auteur]
Laboratoire d'ingénierie des systèmes macromoléculaires [LISM]

Journal title :

Journal of Biomedical Science

Pages :

18

Publisher :

BioMed Central

Publication date :

2024-01-29

ISSN :

1021-7770

English keyword(s) :

Mycobacterial infection
Cystic fibrosis
Hemolytic activity
Pore forming activity
Electron microscopy
Antibiotic resistance
Bactericidal activity
Antimicrobial peptide

HAL domain(s) :

Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Bactériologie
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysique
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses

English abstract : [en]

Mycobacterium abscessus , a fast-growing non-tuberculous mycobacterium, is an emerging opportunistic pathogen responsible for chronic bronchopulmonary infections in people with respiratory diseases such as cystic fibrosis ...
Show more >Mycobacterium abscessus , a fast-growing non-tuberculous mycobacterium, is an emerging opportunistic pathogen responsible for chronic bronchopulmonary infections in people with respiratory diseases such as cystic fibrosis (CF). Due to its intrinsic polyresistance to a wide range of antibiotics, most treatments for M. abscessus pulmonary infections are poorly effective. In this context, antimicrobial peptides (AMPs) active against bacterial strains and less prompt to cause resistance, represent a good alternative to conventional antibiotics. Herein, we evaluated the effect of three arenicin isoforms, possessing two or four Cysteines involved in one ( Ar-1 , Ar-2 ) or two disulfide bonds ( Ar-3 ), on the in vitro growth of M. abscessus . Methods The respective disulfide-free AMPs, were built by replacing the Cysteines with alpha-amino- n -butyric acid (Abu) residue. We evaluated the efficiency of the eight arenicin derivatives through their antimicrobial activity against M. abscessus strains, their cytotoxicity towards human cell lines, and their hemolytic activity on human erythrocytes. The mechanism of action of the Ar-1 peptide was further investigated through membrane permeabilization assay, electron microscopy, lipid insertion assay via surface pressure measurement, and the induction of resistance assay. Results Our results demonstrated that Ar-1 was the safest peptide with no toxicity towards human cells and no hemolytic activity, and the most active against M. abscessus growth. Ar-1 acts by insertion into mycobacterial lipids, resulting in a rapid membranolytic effect that kills M. abscessus without induction of resistance. Conclusion Overall, the present study emphasized Ar-1 as a potential new alternative to conventional antibiotics in the treatment of CF-associated bacterial infection related to M. abscessus .Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL