Title :

Transcriptomic Analysis of Breast Cancer Stem Cells and Development of a pALDH1A1:mNeptune Reporter System for Live Tracking

Author(s) :

Bidan, Nadège [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bailleul-Dubois, Justine [Auteur]
Institut pour la recherche sur le cancer de Lille [Lille] [IRCL]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Duval, Jérémy [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Winter, Marie [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Denoulet, Marie [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Hannebicque, Karine [Auteur]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Institut National de la Santé et de la Recherche Médicale [INSERM]
El-Sayed, Ihsan [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Ginestier, Christophe [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Forissier, Violaine [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Charafe-Jauffret, Emmanuelle [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Macario, Manon [Auteur]
Centre de Recherche en Cancérologie de Marseille [CRCM]
Matsunaga, Yukiko [Auteur]
Université de Lille
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Center for International Research on Integrative Biomedical Systems [University of Tokyo] [CIBiS]
Meignan, Samuel [Auteur]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Institut pour la recherche sur le cancer de Lille [Lille] [IRCL]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Anquez, François [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Julien, Sylvain [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bonnefond, Amelie [Auteur]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Derhourhi, Mehdi [Auteur]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Le Bourhis, Xuefen [Auteur]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 [EGENODIA (GI3M)]
Université Lille Nord de France (COMUE)
Lagadec (Admin), Chann [Auteur correspondant]
Institut pour la recherche sur le cancer de Lille [Lille] [IRCL]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Plasticité Cellulaire et Cancer - U908 [CPAC]

Journal title :

Proteomics

Pages :

1800454

Publisher :

Wiley-VCH Verlag

Publication date :

2019-09-12

ISSN :

1615-9853

English keyword(s) :

ALDH1A1
breast cancer
cancer stem cells
fluorescent reporter systems

HAL domain(s) :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Cancer
Sciences du Vivant [q-bio]/Médecine humaine et pathologie

English abstract : [en]

Many solid cancers are hierarchically organized with a small number of cancer stem cells (CSCs) able to regrow a tumor, while their progeny lacks this feature. Breast CSC is known to contribute to therapy resistance. The ...
Show more >Many solid cancers are hierarchically organized with a small number of cancer stem cells (CSCs) able to regrow a tumor, while their progeny lacks this feature. Breast CSC is known to contribute to therapy resistance. The study of those cells is usually based on their cell-surface markers like CD44high /CD24low/neg or their aldehyde dehydrogenase (ALDH) activity. However, these markers cannot be used to track the dynamics of CSC. Here, a transcriptomic analysis is performed to identify segregating gene expression in CSCs and non-CSCs, sorted by Aldefluor assay. It is observed that among ALDH-associated genes, only ALDH1A1 isoform is increased in CSCs. A CSC reporter system is then developed by using a far red-fluorescent protein (mNeptune) under the control of ALDH1A1 promoter. mNeptune-positive cells exhibit higher sphere-forming capacity, tumor formation, and increased resistance to anticancer therapies. These results indicate that the reporter identifies cells with stemness characteristics. Moreover, live tracking of cells in a microfluidic system reveals a higher extravasation potential of CSCs. Live tracking of non-CSCs under irradiation treatment show, for the first time, live reprogramming of non-CSCs into CSCs. Therefore, the reporter will allow for cell tracking to better understand the implication of CSCs in breast cancer development and recurrence.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL

Submission date :

2024-02-17T04:32:16Z