Competence remodels the pneumococcal cell ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Title :
Competence remodels the pneumococcal cell wall exposing key surface virulence factors that mediate increased host adherence
Author(s) :
Minhas, Vikrant [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Domenech, Arnau [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Synefiaridou, Dimitra [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Straume, Daniel [Auteur]
Norwegian University of Life Sciences [NMBU]
Brendel, Max [Auteur]
Universität Greifswald - University of Greifswald
Cebrero, Gonzalo [Auteur]
Université de Bâle = University of Basel = Basel Universität [Unibas]
Liu, Xue [Auteur]
Shenzhen University [Shenzhen]
Université de Lausanne = University of Lausanne [UNIL]
Costa, Charlotte [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Baldry, Mara [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Sirard, Jean-Claude [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Perez, Camilo [Auteur]
Université de Bâle = University of Basel = Basel Universität [Unibas]
Gisch, Nicolas [Auteur]
Forschungszentrum Borstel, Leibniz Lungenzentrum - Research Center Borstel, Leibniz Lung Center [Sülfeld, Germany]
Hammerschmidt, Sven [Auteur]
Universität Greifswald - University of Greifswald
Håvarstein, Leiv Sigve [Auteur correspondant]
Norwegian University of Life Sciences [NMBU]
Veening, Jan-Willem [Auteur correspondant]
Université de Lausanne = University of Lausanne [UNIL]
Université de Lausanne = University of Lausanne [UNIL]
Domenech, Arnau [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Synefiaridou, Dimitra [Auteur]
Université de Lausanne = University of Lausanne [UNIL]
Straume, Daniel [Auteur]
Norwegian University of Life Sciences [NMBU]
Brendel, Max [Auteur]
Universität Greifswald - University of Greifswald
Cebrero, Gonzalo [Auteur]
Université de Bâle = University of Basel = Basel Universität [Unibas]
Liu, Xue [Auteur]
Shenzhen University [Shenzhen]
Université de Lausanne = University of Lausanne [UNIL]
Costa, Charlotte [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Baldry, Mara [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Sirard, Jean-Claude [Auteur]

Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Perez, Camilo [Auteur]
Université de Bâle = University of Basel = Basel Universität [Unibas]
Gisch, Nicolas [Auteur]
Forschungszentrum Borstel, Leibniz Lungenzentrum - Research Center Borstel, Leibniz Lung Center [Sülfeld, Germany]
Hammerschmidt, Sven [Auteur]
Universität Greifswald - University of Greifswald
Håvarstein, Leiv Sigve [Auteur correspondant]
Norwegian University of Life Sciences [NMBU]
Veening, Jan-Willem [Auteur correspondant]
Université de Lausanne = University of Lausanne [UNIL]
Journal title :
PLoS Biology
Pages :
e3001990
Publisher :
Public Library of Science
Publication date :
2023-01-30
ISSN :
1544-9173
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Competence development in the human pathogen Streptococcus pneumoniae controls several features such as genetic transformation, biofilm formation, and virulence. Competent bacteria produce so-called “fratricins” such as ...
Show more >Competence development in the human pathogen Streptococcus pneumoniae controls several features such as genetic transformation, biofilm formation, and virulence. Competent bacteria produce so-called “fratricins” such as CbpD that kill noncompetent siblings by cleaving peptidoglycan (PGN). CbpD is a choline-binding protein (CBP) that binds to phosphorylcholine residues found on wall and lipoteichoic acids (WTA and LTA) that together with PGN are major constituents of the pneumococcal cell wall. Competent pneumococci are protected against fratricide by producing the immunity protein ComM. How competence and fratricide contribute to virulence is unknown. Here, using a genome-wide CRISPRi-seq screen, we show that genes involved in teichoic acid (TA) biosynthesis are essential during competence. We demonstrate that LytR is the major enzyme mediating the final step in WTA formation, and that, together with ComM, is essential for immunity against CbpD. Importantly, we show that key virulence factors PspA and PspC become more surface-exposed at midcell during competence, in a CbpD-dependent manner. Together, our work supports a model in which activation of competence is crucial for host adherence by increased surface exposure of its various CBPs.Show less >
Show more >Competence development in the human pathogen Streptococcus pneumoniae controls several features such as genetic transformation, biofilm formation, and virulence. Competent bacteria produce so-called “fratricins” such as CbpD that kill noncompetent siblings by cleaving peptidoglycan (PGN). CbpD is a choline-binding protein (CBP) that binds to phosphorylcholine residues found on wall and lipoteichoic acids (WTA and LTA) that together with PGN are major constituents of the pneumococcal cell wall. Competent pneumococci are protected against fratricide by producing the immunity protein ComM. How competence and fratricide contribute to virulence is unknown. Here, using a genome-wide CRISPRi-seq screen, we show that genes involved in teichoic acid (TA) biosynthesis are essential during competence. We demonstrate that LytR is the major enzyme mediating the final step in WTA formation, and that, together with ComM, is essential for immunity against CbpD. Importantly, we show that key virulence factors PspA and PspC become more surface-exposed at midcell during competence, in a CbpD-dependent manner. Together, our work supports a model in which activation of competence is crucial for host adherence by increased surface exposure of its various CBPs.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Source :
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