Titre :

Immunostimulatory Effect of Flagellin on MDR-Klebsiella-Infected Human Airway Epithelial Cells

Auteur(s) :

Van Linge, Christine [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Hulme, Katina [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Peters-Sengers, Hessel [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Sirard, Jean-Claude [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Goessens, Wil [Auteur]
Erasmus University Medical Center [Rotterdam] [Erasmus MC]
de Jong, Menno [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Russell, Colin [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
Boston University [Boston] [BU]
de Vos, Alex [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]
van der Poll, Tom [Auteur]
Amsterdam University Medical Centers [Amsterdam UMC]

Titre de la revue :

International Journal of Molecular Sciences

Pagination :

309

Éditeur :

MDPI

Date de publication :

2023-12-25

ISSN :

1661-6596

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Pneumonia caused by multi-drug-resistant Klebsiella pneumoniae (MDR-Kpneu) poses a major public health threat, especially to immunocompromised or hospitalized patients. This study aimed to determine the immunostimulatory ...
Lire la suite >Pneumonia caused by multi-drug-resistant Klebsiella pneumoniae (MDR-Kpneu) poses a major public health threat, especially to immunocompromised or hospitalized patients. This study aimed to determine the immunostimulatory effect of the Toll-like receptor 5 ligand flagellin on primary human lung epithelial cells during infection with MDR-Kpneu. Human bronchial epithelial (HBE) cells, grown on an air–liquid interface, were inoculated with MDR-Kpneu on the apical side and treated during ongoing infection with antibiotics (meropenem) and/or flagellin on the basolateral and apical side, respectively; the antimicrobial and inflammatory effects of flagellin were determined in the presence or absence of meropenem. In the absence of meropenem, flagellin treatment of MDR-Kpneu-infected HBE cells increased the expression of antibacterial defense genes and the secretion of chemokines; moreover, supernatants of flagellin-exposed HBE cells activated blood neutrophils and monocytes. However, in the presence of meropenem, flagellin did not augment these responses compared to meropenem alone. Flagellin did not impact the outgrowth of MDR-Kpneu. Flagellin enhances antimicrobial gene expression and chemokine release by the MDR-Kpneu-infected primary human bronchial epithelium, which is associated with the release of mediators that activate neutrophils and monocytes. Topical flagellin therapy may have potential to boost immune responses in the lung during pneumonia.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL