Title :

Le miR-675 dérivé du long ARN non codant H19 augmente la tumorigénicité et le potentiel métastatique des cellules cancéreuses mammaires en diminuant l'expression de c-Cbl et Cbl-b

Author(s) :

Vennin, Constance [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Spruyt, Nathalie [Auteur]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Dahmani, Fatima [Auteur]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Julien, Sylvain [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Bertucci, François [Auteur]
Département d’Oncologie Médicale [IPC, Marseille]
Finetti, Pascal [Auteur]
Cancérologie [Inserm U599/IPC]
Chassat, Thierry [Auteur]
Institut Pasteur de Lille
Bourette, Roland [Auteur]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]
Le Bourhis, Xuefen [Auteur]
Plasticité Cellulaire et Cancer - U908 [CPAC]
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 [CANTHER]
Adriaenssens, Eric [Auteur correspondant]
Mécanismes de la Tumorigénèse et Thérapies Ciblées - UMR 8161 [M3T]

Journal title :

Oncotarget

Publisher :

Impact journals

Publication date :

2015-10-06

ISSN :

1949-2553

HAL domain(s) :

Sciences du Vivant [q-bio]/Cancer
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]

English abstract : [en]

H19 is a long non-coding RNA precursor of miR-675microRNA. H19 is increasingly described to play key roles in the progression and metastasis of cancers from different tissue origins. We have previously shown that the H19 ...
Show more >H19 is a long non-coding RNA precursor of miR-675microRNA. H19 is increasingly described to play key roles in the progression and metastasis of cancers from different tissue origins. We have previously shown that the H19 gene is activated by growth factors and increases breast cancer cell invasion. In this study, we established H19/miR-675 ectopic expression models of MDA-MB-231 breast cancer cells to further investigate the underlying mechanisms of H19 oncogenic action. We showed that overexpression of H19/miR-675 enhanced the aggressive phenotype of breast cancer cells including increased cell proliferation and migration in vitro, and increased tumor growth and metastasis in vivo. Moreover, we identified ubiquitin ligase E3 family (c-Cbl and Cbl-b) as direct targets of miR-675 in breast cancer cells. Using a luciferase assay, we demonstrated that H19, through its microRNA, decreased both c-Cbl and Cbl-b expression in all breast cancer cell lines tested. Thus, by directly binding c-Cbl and Cbl-b mRNA, miR-675 increased the stability and the activation of EGFR and c-Met, leading to sustained activation of Akt and Erk as well as enhanced cell proliferation and migration. Our data describe a novel mechanism of protumoral action of H19 in breast cancer.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Cancer Heterogeneity, Plasticity and Resistance to Therapies (CANTHER) - UMR 9020 - UMR 1277

Source :

Harvested from HAL